Anti-Deoxyuridine Triphosphatase (DUT, dUTP Pyrophosphatase, dUTPase, Deoxyuridine 5'-triphosphate N

The enzyme deoxyuridine triphosphate nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP, the immediate precursor of thymidine nucleotides. At least 3 isoforms are produced by alternative splicing. Higher mass isoform DUT-M is primarily located in the mitochondria whereas a lower mass isoform DUT-N is found in the nucleus. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. , Recommended Dilution: Western Blot: 0.5-1.0ug/ml, Immunohistochemistry (Paraffin and Formalin): 1.0-2.0ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.