Annexin V-mFluor(TM) Violet 510 conjugate

Ex: 414 nm. Em: 510 nm. Soluble in: Water. Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This fluorescent Annexin V conjugate has spectral properties similar to Horizon V500® (Horizon V500® is the trademark of BD Biosciences). This Annexin V conjugate has the spectral properties similar to those of Pacific Blue® at Ex/Em = ~400/500 nm (Pacific Blue® is the trademark of Invitrogen). The green fluorescent stain is well excited with the Violet Laser at 405 nm, and emits intense green fluorescence at ~500 nm.