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Amplite(TM) Luciferase Reporter Gene Assay Kit *Bright Glow*

ART NR.: ABD-12519 (10 plates)

Hersteller: AAT Bioquest




Anwendungen: Bioluminescence assays, luciferase assays

Lagerung: -20°C

Versand: +20°C

UniProt ID: P08659 | Suche mit UniProt ID

KEGG ID: K06046 | Suche mit KEGG ID


10 plates

438 €

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Bemerkungen: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The advantages of a luciferase assay are the high sensitivity, the absence of luciferase activity inside most of the cell types, the wide dynamic range, rapidity and low cost. The most versatile and common reporter gene is the luciferase of the North American firefly Photinus pyralis. The protein requires no posttranslational modification for enzyme activity. It is not even toxic in high concentration (in vivo) and can be used in pro- and eukaryotic cells. The firefly luciferase catalyzes the bioluminescent oxidation of luciferin in the presence of ATP, magnesium and oxygen. This AmpliteT Luciferase Reporter Gene Assay Kit uses a proprietary luminogenic formulation to quantify luciferase activity in live cells and cell extracts. Our formulation generates a luminescent product that gives strong luminescence upon interaction with luciferase. The kit provides all the essential components with our optimized mix and read assay protocol that is compatible with HTS liquid handling instruments. It has extremely high sensitivity, and can be used for the assays that require demanding sensitivity.


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