Amplite(TM) Fluorimetric L-Alanine Assay Kit

Ex: 571 nm. Em: 585 nm. L-alanine (L-Ala) plays a crucial role as a building block of important proteins. L-alanine is mostly synthesized by the muscle cells from lactic acid and absorbed into blood via the liver. It is converted into pyruvate by glutamic-pyruvic transaminase to enter the metabolic mainstream. L-Ala is critical for the production of glucose and hence blood sugar management, and plays an important role in the immune system and prevention of kidney stones. Insufficiency of L-alanine is usually a sign of poor nutrition, low protein diet as well as stress. AAT Bioquest's AmpliteT Fluorimetric L-Alanine Assay Kit offers a sensitive fluorescent assay for quantifying L-alanine in biological samples. It utilizes an enzyme coupled reaction that releases hydrogen peroxide, which is detected by Quest FluorT L-Alanine Sensor with a fluorescence microplate reader at Ex/Em = 540/590 nm.