Amplite(TM) Fluorimetric Catalase Assay Kit *Red Fluorescence*

Amplite(TM) Fluorimetric Catalase Assay Kit *Red Fluorescence*
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-11306 1 kit (200 assays) -

3 - 8 business days*

279.00€
 
Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living... more
Product information "Amplite(TM) Fluorimetric Catalase Assay Kit *Red Fluorescence*"
Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen. The enzyme is concentrated in the peroxisome subcellular organelles. Hydrogen peroxide is an ROS that is a toxic product of normal aerobic metabolism and pathogenic ROS production involving oxidase and superoxide dismutase reactions. By preventing the excessive buildup of H2O2, catalase allows important cellular processes which produce H2O2 as a by-product to take place safely. The Amplite(TM) Fluorimetric Catalase Assay Kit provides a quick and sensitive method for the measurement of catalase activity. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Catalase reacts with H2O2 to produce water and oxygen (O2). Amplite(TM) Red also reacts with H2O2 to generate a red fluorescent product. Therefore the reduction in fluorescence intensity is proportional to catalase activity. The Amplite(TM) Red substrate used in the assay enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at 576 nm. With the Amplite(TM) Fluorimetric Catalase Assay Kit, we have detected as little as 30 mU/mL catalase in a 100 µL reaction volume.
Supplier: AAT Bioquest
Supplier-Nr: 11306

Properties

Application: Catalase assays
Format: Homog.

Database Information

KEGG ID : K03781 | Matching products

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Signal Word: Warning
Caution
Our products are for laboratory research use only: Not for administration to humans!
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