Aleuria aurantia (AAL) (Biotin) (mushroom)

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Active Conjugate: ~95% , Lectin Biotinylated:, ~90%, Binding Inhibitors: 100-400mM L-fucose, Applications: Suitable for use in Immunohistochemistry, Blots, ELISA and Cell Sorting. Other applications not tested. Recommended Dilutions: 5-20ug/ml, IHC: 10mM HEPES, pH 7.5, 0.15M sodium chloride. , Blot: 10mM Phosphate, pH 7.8, 0.15M sodium chloride, 0.1% Tween 20. Note: For chemiluminescence, concentrations are generally lower. , ELISA: 10mM Phosphate, pH 7.8, 0.15M sodium chloride. , Cell Sorting: 10mM HEPES pH 7.5, 0.15M sodium chloride., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.