Agaricus bisporus (Mushroom) (ABA) (Biotin) (mushroom)

Lectins are derived from the extracts of plants, animals, viruses and microorganisms. They are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: Gal(b1,3)GalNAc Inhibitory Carbohydrate: b-D-galactose (normally). Recognizes the structure Gal-GalNAc-serine (or threonine). Applications: Suitable for use in Agglutination. Other applications not tested. Recommended Dilutions: Agglutination of type A or B cells: 5-10ug, Agglutination of type O cells: 2ug/ml, Agglutination of neuraminidase treated erythrocytes: < 0.5ug/ml, Optimal dilutions to be determined by researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.