5-(N-ethyl-N-isopropyl)-Amiloride

Sodium-hydrogen exchangers (NHE) are involved in maintaining sodium and pH balance in a variety of tissues. They are also known as sodium-hydrogen antiporters and solute carrier family 9 members. 5-(N-ethyl-N-isopropyl)-Amiloride (EIPA) is a potent inhibitor of several NHE isoforms, inhibiting NHE1, NHE2, NHE3, and NHE5 with Ki values of 0.02, 0.5, 2.4, and 0.42 µM, respectively. It less effectively inhibits NHE4 (IC50 >10 µM). EIPA is commonly used at a concentration of 5-10 µM to inhibit cellular HNE activity.Formal Name: 3-amino-N-(aminoiminomethyl)-6-chloro-5-[ethyl(1-methylethyl)amino]-2-pyrazinecarboxamide. CAS Number: 1154-25-2. Synonyms: EIPA, L-593,754, MH 12-43. Molecular Formula: C11H18ClN7O. Formula Weight: 299.8. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml, DMSO: 14 mg/ml, Ethanol: 2 mg/ml. lambdamax: 230, 291, 380 nm. SMILES: ClC1=C(N(CC)C(C)C)N=C(N)C(C(NC(N)=N)=O)=N1. InChi Code: InChI=1S/C11H18ClN7O/c1-4-19(5(2)3)9-7(12)16-6(8(13)17-9)10(20)18-11(14)15/h5H,4H2,1-3H3,(H2,13,17)(H4,14,15,18,20). InChi Key: QDERNBXNXJCIQK-UHFFFAOYSA-N.