2X Blocking Buffer for Infrared Fluorescent Immunoblot Detection

2X Blocking Buffer for Infrared Fluorescent Immunoblot Detection
Item number Size Datasheet Manual SDS Delivery time Quantity Price
MB-070-0050 50 ml - -

6 - 10 business days*

79.00€
 
Product information "2X Blocking Buffer for Infrared Fluorescent Immunoblot Detection"
Supplier: Rockland Immunochemicals
Supplier-Nr: MB-070-0050

Properties

Application: WB

Database Information

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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Customer review for "2X Blocking Buffer for Infrared Fluorescent Immunoblot Detection"
16 May 2019

Helped to reduce background.

Prior to using the Rockland Blocking Buffer for Fluorescent Western Blots, we had been having problems with sensitivity and background. Using conventional milk or BSA blocking left large amounts of background on the LiCOR imaged PVDF membrane. Using the Rockland Blocking Buffer for Fluorescent Western Blots prior to adding primary, we were able to drastically reduce the amount of background in order to see CFTR in Calu-3 cells in addition to other cell types where CFTR protein amount is greatly decreased. I would recommend not adding tween to the blocking media and to make sure to have ample washing steps.

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