15(S)-HETE

15(S)-HETE is an active metabolite of arachidonic acid (Cay-90010, Cay-90010.1, Cay-10006607). It is formed via oxidation of arachidonic acid by 15-lipoxygenase 2 (15-LO-2) in macrophages, as well as the prostate, lung, and skin, but also by 15-LO-1 in airway epithelial cells, eosinophils, and reticulocytes. 15(S)-HETE inhibits the proliferation of HT-29 colorectal and PC3 prostate cancer cells (IC50s = 40 and 30 µM, respectively). It increases kruppel-like factor 10 (KLF10) and decreases B cell lymphoma 2 (Bcl-2) protein levels in HT-29 cells when used at a concentration of 40 µM. 15(S)-HETE (0.1 µM) increases HMG-CoA reductase protein levels in human dermal microvascular endothelial cells (HDMVECs). It increases Rac1 protein levels and induces farnesylation of Rac1 in HDMVECs when used at a concentration of 0.1 µM.Formal Name: 15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid. CAS Number: 54845-95-3. Synonyms: 15(S)-Hydroxyeicosatetraenoic Acid. Molecular Formula: C20H32O3. Formula Weight: 320.5. Purity: >98%. Formulation: (Request formulation change), A solution in ethanol. Solubility: 0.1 M Na2CO3: 2 mg/ml, DMF: Miscible, DMSO: Miscible, Ethanol: Miscible, PBS (pH 7.2): 0.8 mg/ml. lambdamax: 236 nm. SMILES: CCCCC[C@H](O)/C=C/C=C\C/C=C\C/C=C\CCCC(O)=O. InChi Code: InChI=1S/C20H32O3/c1-2-3-13-16-19(21)17-14-11-9-7-5-4-6-8-10-12-15-18-20(22)23/h4-5,8-11,14,17,19,21H,2-3,6-7,12-13,15-16,18H2,1H3,(H,22,23)/b5-4-,10-8-,11-9-,17-14+/t19-/m0/s1. InChi Key: JSFATNQSLKRBCI-VAEKSGALSA-N.