15-Lipoxygenase-2 (human, recombinant)

Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay10011263-100 100 µg -

6 - 10 business days*

642.00€
 
Two types of 15-lipoxygenase (15-LO) have been discovered and characterized, both of which... more
Product information "15-Lipoxygenase-2 (human, recombinant)"
Two types of 15-lipoxygenase (15-LO) have been discovered and characterized, both of which metabolize arachidonic acid (AA) to produce 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE). 15-LO-1 oxygenates AA at both C-15 and C-12, whereas 15-LO-2 exclusively oxygenates C-15 of AA. Human 15-LO-2 has a molecular mass of approximately 76 kDa and exhibits approximately 40% identity to the reticulocyte 15-LO-1. Expression of 15-LO-2 appears to be restricted to prostate, lung, skin, and cornea and may play a role in the normal development of these tissues. The protein levels and enzymatic activity of 15-LO-2 are both down-regulated in prostate cancer compared with normal and benign prostate tissues, implicating a possible protective role for 15-LO-2 against tumor formation. Cayman's 15-LO-2 (human, recombinant) is expressed and purified from E. coli. The purity was determined using gel electrophoresis followed by coomassie staining. 15-LO-2 specific activity was established using arachidonic acid as the substrate and monitoring diene formation by measuring absorbance 236 nm.Synonyms: 15-LOX-2, 15-LO-2. Purity: >85% estimated by SDS-PAGE. Source: Active human recombinant C-terminal His-tagged protein expressed in E. coli. MW: 76 kDa. Formulation: (Request formulation change), PBS, pH 7.5, with 1 mM DTT, 20% glycerol. UniProt Accession #: O15296.
Keywords: 15-LOX-B, 15-LOX-B, Arachidonate 15-lipoxygenase B, Arachidonate 15-lipoxygenase B, Linoleate 13-lipoxygenase 15-LOb, Linoleate 13-lipoxygenase 15-LOb, Arachidonate 15-lipoxygenase type II, Arachidonate 15-lipoxygenase type II, 15-LOX-2 {ECO:0000303|PubMe
Supplier: Cayman Chemical
Supplier-Nr: 10011263

Properties

Application: Enzyme activity
Conjugate: No
MW: 76 kD
Purity: >85% estimated by SDS-PAGE
Format: Solution

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Signal Word: Warning
GHS Hazard Pictograms:
H Phrases: H316, H319
P Phrases: P264, P280, P305+351+338, P332+313, P337+313
Caution
Our products are for laboratory research use only: Not for administration to humans!
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