1-Oxyl-2,2,5,5-tetramethylpyrroline-3-carboxylate NHS ester

1-Oxyl-2,2,5,5-tetramethylpyrroline-3-carboxylate NHS ester (OXYL-1-NHS) is an N-hydroxysuccinimide ester of the nitroxide spin label 2,2,5,5-tetramethyl-3-carboxylpyrrolin-1-oxyl, also known as tempyo. This ester is an active acylating agent that preferentially targets alpha-amino groups. This reagent can be used to spin label proteins and aminoacylated RNA species.Formal Name: 3-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]-2,5-dihydro-2,2,5,5-tetramethyl-1H-pyrrol-1-yloxy. CAS Number: 37558-29-5. Synonyms: NSC 319633, OXYL-1-NHS, 1-Oxyl-2,2,5,5-tetramethylpyrroline-3-carboxylate N-hydroxysuccinimide ester, Tempyo Ester. Molecular Formula: C13H17N2O5. Formula Weight: 281.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMSO: 20 mg/ml, DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml, Ethanol: 0.3 mg/ml. lambdamax: 219 nm. SMILES: O=C(CC1)N(OC(C2=CC(C)(C)N([O])C2(C)C)=O)C1=O. InChi Code: InChI=1S/C13H17N2O5/c1-12(2)7-8(13(3,4)15(12)19)11(18)20-14-9(16)5-6-10(14)17/h7H,5-6H2,1-4H3. InChi Key: IWVOZPVFXICWQH-UHFFFAOYSA-N.