/ USBio Product-Datasheet T8050-84C

Biomol GmbH
Waidmannstr. 35
22769 HAMBURG
info@biomol.de
www.biomol.de
Tel:+49-40 - 85 32 600
0800 - 24 66 651 free call
Fax: +49-40 - 85 32 60-22
0800 - 24 66 652 free call

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B FC
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120kD.
Catalog #T8050-84C
ApplicationsSuitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended DilutionsFlow Cytometry (Intracellular): 0.5-2ug per 10e6 cells
Flow Cytometry (Cell surface): see Xu et al. (2006)
Western Blot: 1-3ug/ml unconjugated antibody
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D83
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS pH7.2, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa750-850 of the cytoplasmic portion of human TLR8 (Toll-like receptor 8).
SpecificityRecognizes human TLR8 at ~120kD. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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