/ USBio Product-Datasheet T5595-6423

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Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Catalog #T5595-6423
Applications Electrophoresis, Western Blotting and Immunoprecipitation
Enzymatic Activity
Protein-DNA Interaction
Protein-Protein Interaction
Tissue-Specific Expression Identification
Additional Data Supplied All tissue protein samples are supplied with verified donor age and sex.
PreparationTotal protein is prepared from whole-tissue homogenates, immediately frozen in liquid nitrogen after excision, and then stored at -70°C.
Quality Control1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
FormSupplied as a liquid in HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, glycerol, sodium deoxycholate, NP-40, a cocktail of protease inhibitors.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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