/ USBio Product-Datasheet S1013-57D

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Specifications

Siglecs are sialic acid specific I­t ype lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N­terminal Ig ­like V­set domain followed by varying numbers of Ig­like C2­set domains (1, 2). Mouse Siglec­1, also known as sialoadhesin and CD169, is a 175 ­185kD glycoprotein that consists of a 1619 amino acid (aa) extracellular domain (ECD) with one Ig­like V­set domain and 16 Ig­like C2­set domains, a 21 aa transmembrane segment , and a 35 aa cytoplasmic domain (3, 4). Within the ECD, mouse Siglec­1 shares 73% and 83% aa sequence identity with human and rat Siglec­1, respectively. Alternate splicing generates a soluble form of the ECD and a soluble isoform that is truncated following the first three Ig­like domains (3). Siglec­1 expression is restricted to lymphnode and spleen macrophages and some tissue macrophages (4). The adhesive function of Siglec­1 is supported by the N­terminal Ig ­like domain which shows a selectivity for ­2, 3­linked sialic acid residues (4­6). Siglec­1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL­1, and different glycoforms of CD43 (7­10) . Its binding capacity can be masked by endogenous sialylated molecules (11, 12). The sialylated and sulfated N­linked carbohydrates that modify Siglec­1 itself are required for ligand binding (7,8). Siglec­1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (13). It is also induced on circulating monocytes during systemic sclerosis and HI V­1 infection (14­16). Siglec­1 can trap HI V­1 particles for trans infection of permissive cells (15).
Catalog #S1013-57D
Source Murine myeloma cell line, NS0-derived Mouse Siglec1 (Thr20 Arg1639)Accession# NP_035556, IEGRMDP, Mouse IgG2a (Glu98 Lys330)
[N-terminus___________ ____________________________________________C-terminus]
N-terminal Sequence Analysis Thr20
Structure: Disulfidelinked homodimer
Predicted Molecular Mass 201.7kD (monomer)
SDS-PAGE: 190-205kD under reducing conditions
ActivityMeasured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED50 for this effect is typically ~0.6ug/ml.
Endotoxin 1.0 EU per 1ug of the protein by the LAL method.
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and Stability12 months from date of receipt, 20 to 70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, 20 to 70°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Purity90% by SDS­PAGE under reducing conditions and visualized by silver stain.
Concentration~0.5mg/ml
FormLyophilized from a 0.2um filtered solution in PBS. Reconstitute at 100ug/ml in PBS.
SpecificityRecognizes mouse Siglec-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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