/ USBio Product-Datasheet R2079-22

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Catalog #R2079-22
siRNA from United States Biologicalcontrol siRNA oligos
validated siRNA oligos
general siRNA oligos
chemically modified siRNA oligos
fluorescently labeled siRNA oligos
Simple to usedilution buffer included
use your favorite transfection reagent
ApplicationsGene silencing
Genomic chromatin structure determination
Preparation of SolutionAdd 150ul DEPC water to each 1 OD tube to make 20uM solution.
General Handling InstructionsRNA oligonucleotides are susceptible to degradation by exogenous ribonucleases introduced during handling. As a precaution, your siRNs should not be handled with ungloved hands. RNase-free reagents, barrier pipette tips, and tubes should be used. Upon receipt, your RNA oligonucleotides may be safely stored in a non-frost-free freezer for 1 year at or below -20°C. For long term storage, RNA oligonucleotides may be stored at or below -70°C.
Resuspension InstructionsAnnealed siRNAs Briefly centrifuge the tube to ensure that the dried siRNA is at the bottom of the tube. Resuspend siRNA at a convenient concentration. For example, resuspend 2.5 nmole of siRNA in 150ul of the DEPC water provided for a final concentration of 20uM. Once reconstituted in DEPC water, the siRNA is ready to transfect and can be used at your choice of final concentration (e.g., 1-100nM). Store the resuspended siRNA at or below -20°C. Do not store in a frost-free freezer. For long term storage, RNA oligonucleotides may be stored at or below -70°C.
Table 1. Recommended siRNA Resuspension Volumes and Concentrations. siRNA Amount (nmole) DEPC Water to be Added (ml) Final siRNA Concentration (uM) 2.5 0.15 20 5.0 0.25 20 10 0.50 20 20 1.00 20 50 1.00 50 100 1.00 100 150 1.00 150 200 1.00 200
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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