Anti-Nebulin (NEB, DKFZp686C1456, FLJ39568, FLJ39584, NEB177D, NEM2) / USBio Product-Datasheet N0570-74 |
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Biomol GmbH Waidmannstr. 35 22769 HAMBURG info@biomol.de www.biomol.de |
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Anti-Nebulin (NEB, DKFZp686C1456, FLJ39568, FLJ39584, NEB177D, NEM2) |
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| Nebulin is a high molecular weight protein (500-800kD), localized at the N2-line of skeletal muscle myofibrils. It exhibits an elastic stretch dependence, as it maintains the same proportional distance between the z-line and the m-line as the sarcomere is stretched. | |
| Catalog # | N0570-74 |
| Applications | Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. |
| Recommended Dilution | Immunofluorescence (IC): 1:400 shows a typical striated staining pattern of frozen sections of chicken skeletal muscle. It stains within the I-band and shows a decoration line at a distance of 0.22um from the z-line in electron micrographs. |
| Western Blot: 1:100-1:200 detects a band at ~500kD | |
| Optimal dilutions to be determined by the researcher. | |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 9A16 |
| Host | Mouse |
| Source | Chicken |
| Concentration | Not determined |
| Form | Supplied as a liquid, 15mM sodium azide. |
| Purity | Ascites |
| Immunogen | Purified, full-length, native chicken Nebulin. Cellular Localization: Cytoplasmic |
| Specificity | Recognizes chicken Nebulin. Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence. |
| Species crossreactivity | Human, mouse, rat, alligator, amphibian, avian, bovine, fish, porcine, rabbit. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
Questions or comments about this product? Inquire here. |
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| Download Specifications for Anti -Nebulin (NEB, DKFZp686C1456, FLJ39568, FLJ39584, NEB177D, NEM2) |
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