/ USBio Product-Datasheet M2250

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Specifications

Mannan-binding protein (MBP) is a member of the collectin family, the serum proteins, bovine conglutinin and bovine collectin 43 (CL43), and two lung surfactant proteins (SP-A and SP-D). The collectins bind to terminal non-reducing sugar residues, mannose, GlcNAc, fucose and glucose. SP-D binds to maltose. They play important roles in innate immunity without involvement of antibodies (1). The collectins are characterized by their unusual primary structures and also by their unique three-dimensional structures.
Catalog #M2250
MBP, conglutinin, and CL-43 are synthesized in liver and secreted into the circulation. SP-A and SP-D are mainly synthesized in the lung type II alveolar cells and Clara cells, and secreted into the alveolar space. The serum MBP content increases significantly after birth, and their levels vary greatly even among healthy adult individuals. The serum level increases after infection and remains elevated for longer periods compared with other typical acute phase reactants. The collectins have a consistent feature in the gene organization that the signal peptide, N-terminal segment, and the first few Gly-Xaa-Yaa triplets are encoded by a single exon. The rest of the collagen-like sequences are encoded by one (MBL and SP-A) to four (SP-D and conglutinin) exons. The neck region and CRD are each encoded by a single exon (2).
All the known human collectin genes have been found clustered on chromosome 10q22-23. MBP can activate the complement system through the classical pathway (3). Upon binding to carbohydrate structures on the surface of microorganism, the lectin activates the Clr2Cls2 protease complex, like C1q (the overall structure of MBP is very similar to that of C1q), or a novel C1s-like protease, MBP-associated serine proteases (MASPs). These proteases, in turn, activate C2 and C4 of the classical complement pathway and bring about the killing and clearance of the targets. This MBP-mediated complement activation, called the lectin pathway, provides an additional mechanism of microorganism recognition by the complement system in the absence of specific antibodies. Three point mutations in the first exon of the human MBP gene result in MBP deficiency, which is associated with frequent infections in childhood and possibly also in adult.
Recombinant protein corresponding to aa21-248 of human MBL expressed in NSO cells (AAH96182).
Molecular MassThe recombinant mature human MBL, generated by proteolytic removal of the signal peptide, is a monomeric protein which may be self-associated into higher order multimers. Based on N-terminal sequencing, the recombinant human MBL begins with Glu 21 and has a calculated molecular mass of approximately 24kD. The recombinant human MBL migrates as an ~29-33kD protein in SDS-PAGE under reducing conditions.
ActivityMeasured by its ability to bind with Mannan in ELISA.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~29-33kD
SourceMouse myeloma cell line, NS0.
Purity~ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL).
FormSupplied as a lyophilzed powder from PBS, 5% trehalose, 50ug BSA/ug cytokine. Reconstitute with PBS, 0.1% HSA or BSA to 100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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