Anti-Histone H3, dimethyl (Lys79) (Histone H3.1) / USBio Product-Datasheet H5110-12W
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Anti-Histone H3, dimethyl (Lys79) (Histone H3.1)

Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1) and WD-40 domains (WDR5) (5-8). The recent discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic mark (9).
Catalog #H5110-12W
ApplicationsSuitable for use in Western Blot and Chromatin IP. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Chromatin IP: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Numbern/a
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1040
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
PurityPurified
ImmunogenSynthetic peptide containing histone H3 di-methyl lysine 79.
SpecificityRecognizes endogenous levels of histone H3 when di-methylated on Lys79. Species Crossreactivity: human, monkey, mouse, rat.
Does not react with non-methylated, mono-methylated or tri-methylated Lys79. Does not react with methylated histone H3 Lys4, Lys9, Lys27, Lys36 or methylated histone H4 Lys20.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDi-Methyl-Histon e H3, Histone H3/a, HIST1H3A, H3FA, Histone H3/b, HIST1H3B, H3FL, Histone H3/c, HIST1H3C, H3FC, Histone H3/d, HIST1H3D, H3FB, HIST1H3E, H3FD, Histone H3/f, HIST1H3F, H3FI, HIST1H3G, H3FH, Histone H3/h, HIST1H3H, H3FK, Histone H3/i, HIST1H3


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