Cellular Phosphotyrosine ELISA Kit, BioAssay, / USBio Product-Datasheet C2609-50
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22769 HAMBURG
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Cellular Phosphotyrosine ELISA Kit, BioAssay,

Specifications

The cellular phosphotyrosine direct enzyme-linked immunoabsorbant assay (ELISA) kit can be used to characterize tyrosine phosphorylation in adherent cultured cells. It has been shown to be useful for analyzing the effect of inhibitors of cellular tyrosine phosphorylation. This kit requires a model cell line provided by the researcher that can be stimulated in vitro to undergo tyrosine phosphorylation. Human A431 carcinoma cell and foreskin fibroblast cells stimulated with epidermal growth factor as well as mouse 3T3 fibroblasts stimulated with insulin have been successfully used with this kit.
Catalog #C2609-50
ApplicationsCharacterization of tyrosine phosphorylation in adherent cultured cells
Capacity:
192 Assays
Components:
C2609-50A: Phosphotyrosine (HRP) 1x200ug
C2609-50B: Blocking Reagent 1x2g
C2609-50C: TMB tablets 1x4tablets
C2609-50D: Phosphate-Citrate Buffer (5x) 1x8ml
C2609-50E: DMSO 1x4ml
C2609-50F: Tissue culture plates 2x96wells
Other components required but not included as part of kit areFresh Hydrogen Peroxide 30%
Model cell line
Culture media
Growth Factor or cytokine
Phosphate Buffered Saline (PBS), pH 7.4
Protein tyrosine kinase inhibitors
ELISA Plate Reader with filter for single wavelength for 375nm, 450nm, and/or 655nm
37°C incubator
timer
variable volume (5-200l) pipet + tips
5, 10 and 25ml pipets
Acetic acid
Methanol
0.5M H2SO4
Storage and StabilityStable for 6 months at 4°C from date of shipment.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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