/ USBio Product-Datasheet C2409-26

Biomol GmbH
Waidmannstr. 35
22769 HAMBURG
info@biomol.de
www.biomol.de
Tel:+49-40 - 85 32 600
0800 - 24 66 651 free call
Fax: +49-40 - 85 32 60-22
0800 - 24 66 652 free call

Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IP IH FC
ICAM-1 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. ICAM-1 has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells and on a variety of other cell types including activated fibroblasts, EC, macrophages and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. Inhibits adhesion of infected RBCs and LFA-1 to ICAM-1. CD54 is the receptor for rhinoviruses and malaria.
Catalog #C2409-26
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Also suitable for use in studies of inflammation, lymphocyte differentiation and activation. Other applications have not been tested.
Recommended DilutionFlow Cytometry: 1ug to stain 1x10e6 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No1.BB.705
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 2mM EDTA, 0.1% sodium azide. Labeled with biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenCD54 (ICAM-1)
SpecificityRecognizes the Mr 95kD cell surface glycoprotein known as intracellular adhesion molecule ICAM-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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