/ USBio Product-Datasheet C2252-03G

Biomol GmbH
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22769 HAMBURG
info@biomol.de
www.biomol.de
Tel:+49-40 - 85 32 600
0800 - 24 66 651 free call
Fax: +49-40 - 85 32 60-22
0800 - 24 66 652 free call

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
The human CD1 genetic locus consists of five closely related genes. Three of the gene products (CD1a, CD1b and CD1c) have been officially clustered using antibodies and are expressed on cortical thymocytes, a subset of B cells, and on most professional antigen presenting cells. CD1 molecules have a specialized lipid antigen-presenting function and it is unlikely that they present conventional peptide antigens. CD1 may also be involved in costimulation.
Catalog #C2252-03G
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 5ug/ml
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Five x 10e5 cultured Molt-4 cells per tube were washed and incubated 45 minutes on ice with 80ul C2252-03G at
5ug/ml.
2. Cells were washed twice and incubated with secondary reagent Streptavidin/R-Phycoerythrin (S7973-88P), after which they were washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 1.82 log 10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration.
4. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5ug/ml anti-CD1c antibody.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No7H208
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman T cell line Molt 4.
SpecificityRecognizes the human CD1c molecule of ~48kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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