/ USBio Product-Datasheet 0004-01H

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22769 HAMBURG
info@biomol.de
www.biomol.de
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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
TNFSF9, a type II transmembrane glycoprotein and member of the TNF superfamily, preferentially expressed by B cells, which acts as a ligand for CD137 (4-1BB ligand receptor/TNFRSF9). Interaction between 4-1BBL and its receptor acts as a co-stimulatory signal for T cell activation and expansion, and is involved in the generation of cytotoxic T cells (Tc) and in the process of antigen presentation. Expression of 4-1BBL by a variety of carcinoma cells, suggests a role for 4-1BBL in tumor cell-T cell interactions.
Catalog #0004-01H
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.5-2ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesGoat Anti Rabbit IgG (H&L) HRP
Sheep Anti Rabbit IgG Biotin
Goat Anti Rabbit IgG (Fc) FITC, HRP
Goat Anti Rabbit IgG (H&L) HRP
Sheep Anti Rabbit IgG FITC
Sheep Anti Rabbit IgG RPE
Sheep Anti Rabbit IgG DyLight488, DyLight549, DyLight649
Sheep Anti Rabbit IgG HRP
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 100ul dH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For long term storage the addition of 0.09% sodium azide is recommended. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in 100ul distilled water.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant human 4-1BBL.
SpecificityRecognizes human 4-1BB Ligand, otherwise known as TNFSF9
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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