MaxTag Anti-Goat IgG Kit for Immunoblotting

Art Nr.: KIA004, Rockland
Biomol GmbH
Waidmannstr. 35
22769 HAMBURG
info@biomol.de
www.biomol.de
Tel:+49-40 - 85 32 600
0800 - 24 66 651 free call
Fax: +49-40 - 85 32 60-22
0800 - 24 66 652 free call

Colorimetric WBlot Kit: TMB/DAB Substrate Kit for WBlot using Goat Primary Ab MaxTag™ Properties



Description Colorimetric Western Blot Kit: TMB/DAB Substrate Kit for Western Blot using Goat Primary Antibody MaxTag™ - KIA004

Known Cross Reactivity Anti-Goat IgG

Application Western Blot : 1:2,000

Product Type Kit

Kit Type Colorimetric Western Blot Kit

Physical State n/a

Shipping condition Ambient

Label Unconjugated

Concentration Sufficient to run approximately 40 immunoblots (10 cm x 10 cm).

Preservative Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

Related Links Colorimetric Western Blot Kit: Goat Primary Antibodies Protocol  

Colorimetric WBlot Kit: TMB/DAB Substrate Kit for WBlot using Goat Primary Ab MaxTag™ Description

Background

This western blotting kit allows for the detection of primary goat polyclonal antibody provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. The first step in the detection of the membrane bound primary antibody-antigen complex is the addition of a secondary antibody that has been affinity purified, cross-adsorbed, and biotinylated. This biotinylated secondary antibody is visualized after reaction with streptavidin-peroxidase conjugate and subsequent addition of either TMB or DAB substrates. The increased sensitivity and low background achieved by Rockland Immunochemicals MaxTag? Western Blotting Kit relies on the use of an enhanced streptavidin-peroxidase conjugate and a highly active biotinylated antibody. The streptavidin carries a neutral charge and fewer carbohydrate groups than positively charged avidin. The biotin incorporates a spacer group that allows for better accessibility of the streptavidin-peroxidase conjugate.

Storage Condition

See kit insert for complete instructions.

Application Note

Rockland Immunochemicals MaxTag? western blotting kit provides a rapid, sensitive, and economical way to develop western blots. The design of this kit simplifies the process of western blotting applications. Straightforward procedures and color-coding add to the ease of use. Two substrates are provided for flexibility, TMB substrate producing a bright blue color and DAB substrate producing a dark brown color. This kit can process up to 40 blots (100 cm2) and is stable for at least 1 year when stored at +4°C.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Specific Reference

close