Anti-Mouse (Kappa Chain) [Rabbit] Rhodamine conjugated / Rockland Product-Datasheet 610-4010

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Anti-Mouse (Kappa Chain) [Rabbit] Rhodamine conjugated
Code: 610-4010
Size: 1 mg

Mouse κ (kappa chain) Antibody Rhodamine Conjugated Properties



Description Anti-MOUSE κ (kappa chain) (RABBIT) Antibody Rhodamine Conjugated - 610-4010

Host Rabbit

Specificity κ (kappa chain)

Target Species Mouse

Clonality Polyclonal

Application FLISA : 1:10,000 - 1:50,000
IF Microscopy : 1:1,000 - 1:5,000
FlowCytometry : 1:500 - 1:2,500
Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500

Physical State Lyophilized

Shipping condition Ambient

Format IgG

Label Rhodamine (TRITC)

FP Value 3.8 moles Rhodamine (TRITC) per mole of IgG

Concentration 1.0 mg/mL by UV absorbance at 280 nm

Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume 1.0 mL

Reconstitution Buffer Restore with deionized water (or equivalent)

Preservative 0.01% (w/v) Sodium Azide

Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Download Datasheet Download CofA  

Mouse κ (kappa chain) Antibody Rhodamine Conjugated Description

Background

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Immunogen

Mouse kappa light chain

Storage Condition

Store vial at 4 °C prior to restoration.   For extended storage aliquot contents and freeze at -20 °C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4 °C as an undiluted liquid.  Dilute only prior to immediate use. 

Purity/Specifity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.   Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Specific Reference


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