Anti-Glutamate Dehydrogenase (bovine liver) (GDH), Horseradish Peroxidase conjugated / Rockland Product-Datasheet 200-4358

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Anti-Glutamate Dehydrogenase (bovine liver) (GDH), Horseradish Peroxidase conjugated
Code: 200-4358
Size: 20 mg

Glutamate Dehydrogenase Antibody Peroxidase Conjugated Properties

Description
Anti-GLUTAMATE DEHYDROGENASE (Bovine Liver) (RABBIT) Antibody Peroxidase Conjugated - 200-4358
Host
Rabbit
Target Species
Bovine
Known Cross Reactivity
bovine
Clonality
Polyclonal
Application
ELISA : 1:5,000 - 1:20,000
Western Blot : 1:500 - 1:5,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
Peroxidase (Horseradish)
Concentration
10.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
2.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
GLUD1
Related Links
UniProtKBNCBINCBI - 32880221UniProt - P00366Gene ID - 281785
Download Datasheet
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Glutamate Dehydrogenase Antibody Peroxidase Conjugated Description

Immunogen
Glutamate Dehydrogenase [Bovine Liver]
Immunogen Type
Native Protein
Storage Condition
Store vial at 4 °C prior to restoration.   For extended storage aliquot contents and freeze at -20 °C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4 °C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
This product has been assayed against 1.0 ug of Glutamate Dehydrogenase [Bovine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.  This product has been assayed against 1.0 ug of Glutamate Dehydrogenase [Bovine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.  This product has been assayed against 1.0 ug of Glutamate Dehydrogenase [Bovine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. 
Purity/Specifity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver].  Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference


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