Anti-MAP2K7, clone 4E5 / Product-Datasheet Pro-30291 |
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Biomol GmbH Waidmannstr. 35 22769 HAMBURG info@biomol.de www.biomol.de |
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Anti-MAP2K7, clone 4E5 |
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| Specification | |
|---|---|
| Entrez GeneID | 5609 |
| Aliases | MKK7; Jnkk2; MAPKK7; PRKMK7 |
| Clone# | 4E5 |
| MW | 48kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | 4℃; -20℃ for long term storage |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAP2K7 expressed in E. Coli.
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| Formulation | Ascitic fluid containing 0.03% sodium azide.
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| Application | |
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| ICC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
| Description |
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The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.
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| References |
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| 1. Biochem J. 2010 Mar 29;427(2):237-45.
2. J Immunol. 2008 Sep 1;181(5):3252-8. |

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);

Figure 1: Western blot analysis using MAP2K7 mAb against human MAP2K7 (AA: 7-178) recombinant protein. (Expected MW is 45.1 kDa)

Figure 5: Flow cytometric analysis of Hela cells using MAP2K7 mouse mAb (green) and negative control (red).

Figure 4: Immunofluorescence analysis of Hela cells using MAP2K7 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MAP2K7 mouse mAb with DAB staining.

Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K7 mouse mAb with DAB staining.
| Tissue Array Results | |||||
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| Position | Tissues Name | Result | Localization | ||
| Nucleus | Cytoplasm | Membrane | |||
| A1/2 | Esophageal Cancer | ||||
| B1/2 | Esophageal Cancer | ||||
| C1/2 | Esophagus | ||||
| D1/2 | Esophagus | ||||
| E1/2 | Stomach Cancer | ||||
| F1/2 | Stomach Cancer | ||||
| G1/2 | Stomach | ||||
| H1/2 | Stomach | ||||
| A3/4 | Colon Cancer | ||||
| B3/4 | Colon Cancer | ||||
| C3/4 | Colon | ||||
| D3/4 | Duodenum | ||||
| E3/4 | Rectal Cancer | ||||
| F3/4 | Rectal Cancer | ||||
| G3/4 | Rectum | ||||
| H3/4 | Testis | ||||
| A5/6 | Hepatic Carcinoma | ||||
| B5/6 | Hepatic Carcinoma | ||||
| C5/6 | Liver | ||||
| D5/6 | Renal cell Carcinoma | ||||
| E5/6 | Kidney | ||||
| F5/6 | Pancreas | ||||
| G5/6 | Prostate Cancer | ||||
| H5/6 | Lymphoid tissue | ||||
| A7/8 | Lung Cancer | ||||
| B7/8 | Lung Cancer | ||||
| C7/8 | Lung | ||||
| D7/8 | Brain | ||||
| E7/8 | Brain | ||||
| F7/8 | Medulla oblongata | ||||
| G7/8 | Cerebellar | ||||
| H7/8 | Skeletal muscle | ||||
| A9/10 | Breast Cancer | ||||
| B9/10 | Breast Cancer | ||||
| C9/10 | Ovarian Cancer | ||||
| D9/10 | Ovarian Cancer | ||||
| E9/10 | Endometrial Cancer | ||||
| F9/10 | Cervical Cancer | ||||
| G9/10 | Bladder Cancer | ||||
| H9/10 | Myocardial | ||||