Anti-MAP2K7, clone 4E5 / Product-Datasheet Pro-30291

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Anti-MAP2K7, clone 4E5
Specification
Entrez GeneID 5609
Aliases MKK7; Jnkk2; MAPKK7; PRKMK7
Clone# 4E5
MW 48kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human
Immunogen Purified recombinant fragment of human MAP2K7 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.
References
1. Biochem J. 2010 Mar 29;427(2):237-45.
2. J Immunol. 2008 Sep 1;181(5):3252-8.
Related Products

Elisa

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);

Western Blot

Figure 1: Western blot analysis using MAP2K7 mAb against human MAP2K7 (AA: 7-178) recombinant protein. (Expected MW is 45.1 kDa)

Flow cytometric

Figure 5: Flow cytometric analysis of Hela cells using MAP2K7 mouse mAb (green) and negative control (red).

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of Hela cells using MAP2K7 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MAP2K7 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K7 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer
B1/2 Esophageal Cancer
C1/2 Esophagus
D1/2 Esophagus
E1/2 Stomach Cancer
F1/2 Stomach Cancer
G1/2 Stomach
H1/2 Stomach
A3/4 Colon Cancer
B3/4 Colon Cancer
C3/4 Colon
D3/4 Duodenum
E3/4 Rectal Cancer
F3/4 Rectal Cancer
G3/4 Rectum
H3/4 Testis
A5/6 Hepatic Carcinoma
B5/6 Hepatic Carcinoma
C5/6 Liver
D5/6 Renal cell Carcinoma
E5/6 Kidney
F5/6 Pancreas
G5/6 Prostate Cancer
H5/6 Lymphoid tissue
A7/8 Lung Cancer
B7/8 Lung Cancer
C7/8 Lung
D7/8 Brain
E7/8 Brain
F7/8 Medulla oblongata
G7/8 Cerebellar
H7/8 Skeletal muscle
A9/10 Breast Cancer
B9/10 Breast Cancer
C9/10 Ovarian Cancer
D9/10 Ovarian Cancer
E9/10 Endometrial Cancer
F9/10 Cervical Cancer
G9/10 Bladder Cancer
H9/10 Myocardial


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