Anti-GKAP, clone 3G4 / Product-Datasheet Pro-30238

Biomol GmbH
Waidmannstr. 35
22769 HAMBURG
info@biomol.de
www.biomol.de
Tel:+49-40 - 85 32 600
0800 - 24 66 651 free call
Fax: +49-40 - 85 32 60-22
0800 - 24 66 652 free call
Anti-GKAP, clone 3G4
Specification
Entrez GeneID 9229
Aliases GKAP; DLGAP1; DAP-1; hGKAP; SAPAP1; FLJ38442; MGC88156; DAP-1-BETA; DAP-1-ALPHA
Clone# 3G4
MW 109kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human
Immunogen Purified recombinant fragment of human GKAP expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ICC 1/200 - 1/1000
FCM N/A
ELISA 1/10000
Description
Function: Part of the postsynaptic scaffold in neuronal cells.Tissue specificity: Expressed in brain.
References
1. Am J Hum Genet. 2009 Nov;85(5):628-42.
2. Genes Immun. 2010 Apr;11(3):232-8.
Related Products

Elisa

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);

Western Blot

Figure 1: Western blot analysis using GKAP mAb against HEK293 (1) and GKAP(AA: 490-663)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of NIH/3T3 cells using GKAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using GKAP mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded human esophagus tissues using GKAP mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer
B1/2 Esophageal Cancer
C1/2 Esophagus
D1/2 Esophagus
E1/2 Stomach Cancer
F1/2 Stomach Cancer
G1/2 Stomach
H1/2 Stomach
A3/4 Colon Cancer
B3/4 Colon Cancer
C3/4 Colon
D3/4 Duodenum
E3/4 Rectal Cancer
F3/4 Rectal Cancer
G3/4 Rectum
H3/4 Testis
A5/6 Hepatic Carcinoma
B5/6 Hepatic Carcinoma
C5/6 Liver
D5/6 Renal cell Carcinoma
E5/6 Kidney
F5/6 Pancreas
G5/6 Prostate Cancer
H5/6 Lymphoid tissue
A7/8 Lung Cancer
B7/8 Lung Cancer
C7/8 Lung
D7/8 Brain
E7/8 Brain
F7/8 Medulla oblongata
G7/8 Cerebellar
H7/8 Skeletal muscle
A9/10 Breast Cancer
B9/10 Breast Cancer
C9/10 Ovarian Cancer
D9/10 Ovarian Cancer
E9/10 Endometrial Cancer
F9/10 Cervical Cancer
G9/10 Bladder Cancer
H9/10 Myocardial


Fenster schließen