Anti-GKAP, clone 3G4 / Product-Datasheet Pro-30238 |
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Biomol GmbH Waidmannstr. 35 22769 HAMBURG info@biomol.de www.biomol.de |
Tel:+49-40 - 85 32 600 0800 - 24 66 651 free call Fax: +49-40 - 85 32 60-22 0800 - 24 66 652 free call |
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Anti-GKAP, clone 3G4 |
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| Specification | |
|---|---|
| Entrez GeneID | 9229 |
| Aliases | GKAP; DLGAP1; DAP-1; hGKAP; SAPAP1; FLJ38442; MGC88156; DAP-1-BETA; DAP-1-ALPHA |
| Clone# | 3G4 |
| MW | 109kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | 4℃; -20℃ for long term storage |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GKAP expressed in E. Coli.
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| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Application | |
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| ICC | 1/200 - 1/1000 |
| FCM | N/A |
| ELISA | 1/10000 |
| Description |
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Function: Part of the postsynaptic scaffold in neuronal cells.Tissue specificity: Expressed in brain.
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| References |
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| 1. Am J Hum Genet. 2009 Nov;85(5):628-42.
2. Genes Immun. 2010 Apr;11(3):232-8. |

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);

Figure 1: Western blot analysis using GKAP mAb against HEK293 (1) and GKAP(AA: 490-663)-hIgGFc transfected HEK293 (2) cell lysate.

Figure 4: Immunofluorescence analysis of NIH/3T3 cells using GKAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Figure 2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using GKAP mouse mAb with DAB staining.

Figure 3: Immunohistochemical analysis of paraffin-embedded human esophagus tissues using GKAP mouse mAb with DAB staining.
| Tissue Array Results | |||||
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| Position | Tissues Name | Result | Localization | ||
| Nucleus | Cytoplasm | Membrane | |||
| A1/2 | Esophageal Cancer | ||||
| B1/2 | Esophageal Cancer | ||||
| C1/2 | Esophagus | ||||
| D1/2 | Esophagus | ||||
| E1/2 | Stomach Cancer | ||||
| F1/2 | Stomach Cancer | ||||
| G1/2 | Stomach | ||||
| H1/2 | Stomach | ||||
| A3/4 | Colon Cancer | ||||
| B3/4 | Colon Cancer | ||||
| C3/4 | Colon | ||||
| D3/4 | Duodenum | ||||
| E3/4 | Rectal Cancer | ||||
| F3/4 | Rectal Cancer | ||||
| G3/4 | Rectum | ||||
| H3/4 | Testis | ||||
| A5/6 | Hepatic Carcinoma | ||||
| B5/6 | Hepatic Carcinoma | ||||
| C5/6 | Liver | ||||
| D5/6 | Renal cell Carcinoma | ||||
| E5/6 | Kidney | ||||
| F5/6 | Pancreas | ||||
| G5/6 | Prostate Cancer | ||||
| H5/6 | Lymphoid tissue | ||||
| A7/8 | Lung Cancer | ||||
| B7/8 | Lung Cancer | ||||
| C7/8 | Lung | ||||
| D7/8 | Brain | ||||
| E7/8 | Brain | ||||
| F7/8 | Medulla oblongata | ||||
| G7/8 | Cerebellar | ||||
| H7/8 | Skeletal muscle | ||||
| A9/10 | Breast Cancer | ||||
| B9/10 | Breast Cancer | ||||
| C9/10 | Ovarian Cancer | ||||
| D9/10 | Ovarian Cancer | ||||
| E9/10 | Endometrial Cancer | ||||
| F9/10 | Cervical Cancer | ||||
| G9/10 | Bladder Cancer | ||||
| H9/10 | Myocardial | ||||