Anti-TLR8 (Toll Like Receptor-8), FITC conjugated, clone 6D83Art Nr.: T8050-84C, USBio
Tel:+49-40 - 85 32 600
0800 - 24 66 651 free call
Fax: +49-40 - 85 32 60-22
0800 - 24 66 652 free call
|The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120kD.|
|Applications||Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.|
|Recommended Dilutions||Flow Cytometry (Intracellular): 0.5-2ug per 10e6 cells |
|Flow Cytometry (Cell surface): see Xu et al. (2006)|
|Western Blot: 1-3ug/ml unconjugated antibody|
|Optimal dilution to be determined by researcher.|
|Storage and Stability||May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.|
|Form||Supplied as a liquid in PBS pH7.2, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).|
|Purity||Purified by Protein G affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to aa750-850 of the cytoplasmic portion of human TLR8 (Toll-like receptor 8).|
|Specificity||Recognizes human TLR8 at ~120kD. Species Crossreactivity: mouse.|
|Important Note||This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.|
This product is offered by Biomol for research purposes only.
Not for diagnostic purposes or human use.
It may not be resold or used to manufacture commercial products without written approval of Biomol GmbH.