Anti-Retinoblastoma (Rb, Retinoblastoma-associated Protein, p105-Rb, pRb, pp110, RB1)Art Nr.: R1660-05Q2, USBio
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|RB1 likely acts as a regulator of other genes. It forms a complex with adenovirus E1A and with SV40 large T antigen, acts as a tumor suppressor, and may bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. RB1 is a potent inhibitor of E2F-mediated trans-activation, and also recruits and targets histone methyltransferase SUV39H1 leading to epigenetic transcriptional repression. This protein inhibits the intrinsic kinase activity of TAF1. Defects in RB1 are the cause of childhood cancer retinoblastoma (RB), a congenital malignant tumor that arises from the nuclear layers of the retina. Defects in RB1 are also a cause of bladder cancer and osteogenic sarcoma.|
|Applications||Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.|
|Recommended Dilution||Western Blot:1:500-1:1,000|
|Optimal dilutions to be determined by the researcher.|
|Storage and Stability||May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.|
|Concentration||As reported |
|Form||Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.|
|Purity||Purified by immunoaffinity chromatography.|
|Immunogen||Synthetic non-phosphopeptide corresponding to human Rb around the phosphorylation site of serine 780 (T-L-SP-P-I).|
|Specificity||Recognizes human Retinoblastoma. Species Cross-Reactivity: mouse, rat.|
|Important Note||This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.|
This product is offered by Biomol for research purposes only.
Not for diagnostic purposes or human use.
It may not be resold or used to manufacture commercial products without written approval of Biomol GmbH.