Anti-GKAP, clone 3G4

Art Nr.: Pro-30238, ProMab
Biomol GmbH
Waidmannstr. 35
22769 HAMBURG
info@biomol.de
www.biomol.de
Tel:+49-40 - 85 32 600
0800 - 24 66 651 free call
Fax: +49-40 - 85 32 60-22
0800 - 24 66 652 free call
Specification
Entrez GeneID 9229
Aliases GKAP; DLGAP1; DAP-1; hGKAP; SAPAP1; FLJ38442; MGC88156; DAP-1-BETA; DAP-1-ALPHA
Clone# 3G4
MW 109kDa
Host/Isotype Mouse IgG1
Storage 4?; -20? for long term storage
Species Reactivity Human
Immunogen Purified recombinant fragment of human GKAP expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ICC 1/200 - 1/1000
FCM N/A
ELISA 1/10000
Description
Function: Part of the postsynaptic scaffold in neuronal cells.Tissue specificity: Expressed in brain.
References
1. Am J Hum Genet. 2009 Nov;85(5):628-42.
2. Genes Immun. 2010 Apr;11(3):232-8.
Related Products

Elisa

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);

Western Blot

Figure 1: Western blot analysis using GKAP mAb against HEK293 (1) and GKAP(AA: 490-663)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using GKAP mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer -
C1/2 Esophagus -
D1/2 Esophagus -
E1/2 Stomach Cancer -
F1/2 Stomach Cancer -
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer -
B3/4 Colon Cancer -
C3/4 Colon -
D3/4 Duodenum -
E3/4 Rectal Cancer -
F3/4 Rectal Cancer -
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver -
D5/6 Renal cell Carcinoma -
E5/6 Kidney -
F5/6 Pancreas -
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer +
B7/8 Lung Cancer -
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle +
A9/10 Breast Cancer -
B9/10 Breast Cancer -
C9/10 Ovarian Cancer -
D9/10 Ovarian Cancer -
E9/10 Endometrial Cancer -
F9/10 Cervical Cancer -
G9/10 Bladder Cancer -
H9/10 Myocardial -

This product is offered by Biomol for research purposes only. Not for diagnostic purposes or human use. It may not be resold or used to manufacture commercial products without written approval of Biomol GmbH.

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