|Aliases||MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; MAP2K6|
|Storage||4?; -20? for long term storage|
|Species Reactivity||Human; Mouse|
|Immunogen||Purified recombinant fragment of human MAP2K6 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|WB||1/500 - 1/2000|
|ICC||1/200 - 1/1000|
|FCM||1/200 - 1/400|
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. Tissue specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1, on the other hand, is found in skeletal muscle, heart, and in lesser extent in liver or pancreas .
|1. J Mol Med. 2008 Apr;86(4):485-90.
2. Blood. 2007 Jan 1;109(1):185-93.
Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);
Figure 1: Western blot analysis using MAP2K6 mouse mAb against HepG2 (1), MCF-7 (2) and NIH/3T3 (3) cell lysate.
Figure 2: Immunofluorescence analysis of Hela cells using MAP2K6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Flow cytometric analysis of Hela cells using MAP2K6 mouse mAb (green) and negative control (purple).
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