Anti-MAP2K6, clone 3H12C8

Art Nr.: Pro-30165, ProMab
Biomol GmbH
Waidmannstr. 35
22769 HAMBURG
info@biomol.de
www.biomol.de
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Specification
Entrez GeneID 5608
Aliases MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; MAP2K6
Clone# 3H12C8
MW 38kDa
Host/Isotype Mouse IgG1
Storage 4?; -20? for long term storage
Species Reactivity Human; Mouse
Immunogen Purified recombinant fragment of human MAP2K6 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Application
WB 1/500 - 1/2000
IHC N/A
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. Tissue specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1, on the other hand, is found in skeletal muscle, heart, and in lesser extent in liver or pancreas .
References
1. J Mol Med. 2008 Apr;86(4):485-90.
2. Blood. 2007 Jan 1;109(1):185-93.
Seqence
full(1-334)
Related Products

Elisa

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);

Western Blot

Figure 1: Western blot analysis using MAP2K6 mouse mAb against HepG2 (1), MCF-7 (2) and NIH/3T3 (3) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of Hela cells using MAP2K6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric

Figure 3: Flow cytometric analysis of Hela cells using MAP2K6 mouse mAb (green) and negative control (purple).


This product is offered by Biomol for research purposes only. Not for diagnostic purposes or human use. It may not be resold or used to manufacture commercial products without written approval of Biomol GmbH.

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