Anti-Nebulin (NEB, DKFZp686C1456, FLJ39568, FLJ39584, NEB177D, NEM2)

Art Nr.: N0570-74, USBio
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Clone Host Grade Applications
Monoclonal Mouse Ascites B IC IF
Nebulin is a high molecular weight protein (500-800kD), localized at the N2-line of skeletal muscle myofibrils. It exhibits an elastic stretch dependence, as it maintains the same proportional distance between the z-line and the m-line as the sarcomere is stretched.
Catalog #N0570-74
ApplicationsSuitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunofluorescence (IC): 1:400 shows a typical striated staining pattern of frozen sections of chicken skeletal muscle. It stains within the I-band and shows a decoration line at a distance of 0.22um from the z-line in electron micrographs.
Western Blot: 1:400 detects a band at ~500kD
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No9A16
ConcentrationNot Determined
FormSupplied as a liquid, 15mM sodium azide.
ImmunogenPurified, full-length, native chicken Nebulin. Cellular Localization: Cytoplasmic
SpecificityRecognizes chicken Nebulin. Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence.
Species CrossreactivityHuman, mouse, rat, alligator, amphibian, avian, bovine, fish, porcine and rabbit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

This product is offered by Biomol for research purposes only. Not for diagnostic purposes or human use. It may not be resold or used to manufacture commercial products without written approval of Biomol GmbH.