Colorimetric WBlot Kit: TMB/DAB Substrate Kit for WBlot using Goat Primary Ab MaxTag™ Properties
Colorimetric Western Blot Kit: TMB/DAB Substrate Kit for Western Blot using Goat Primary Antibody MaxTag™ - KIA004
Known Cross Reactivity
Western Blot : 1:2,000
Colorimetric Western Blot Kit
Sufficient to run approximately 40 immunoblots (10 cm x 10 cm).
Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
Colorimetric Western Blot Kit: Goat Primary Antibodies Protocol
Colorimetric WBlot Kit: TMB/DAB Substrate Kit for WBlot using Goat Primary Ab MaxTag™ Description
This western blotting kit allows for the detection of primary goat polyclonal antibody provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. The first step in the detection of the membrane bound primary antibody-antigen complex is the addition of a secondary antibody that has been affinity purified, cross-adsorbed, and biotinylated. This biotinylated secondary antibody is visualized after reaction with streptavidin-peroxidase conjugate and subsequent addition of either TMB or DAB substrates.
The increased sensitivity and low background achieved by Rockland Immunochemicals MaxTag? Western Blotting Kit relies on the use of an enhanced streptavidin-peroxidase conjugate and a highly active biotinylated antibody. The streptavidin carries a neutral charge and fewer carbohydrate groups than positively charged avidin. The biotin incorporates a spacer group that allows for better accessibility of the streptavidin-peroxidase conjugate.
See kit insert for complete instructions.
Rockland Immunochemicals MaxTag? western blotting kit provides a rapid, sensitive, and economical way to develop western blots. The design of this kit simplifies the process of western blotting applications. Straightforward procedures and color-coding add to the ease of use. Two substrates are provided for flexibility, TMB substrate producing a bright blue color and DAB substrate producing a dark brown color. This kit can process up to 40 blots (100 cm2) and is stable for at least 1 year when stored at +4°C.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
This product is offered by Biomol for research purposes only.
Not for diagnostic purposes or human use.
It may not be resold or used to manufacture commercial products without written approval of Biomol GmbH.