Anti-Cyclin B1, clone 0.N.237

Art Nr.: C8453-10, USBio
Biomol GmbH
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22769 HAMBURG
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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Catalog #C8453-10
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipation. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2 hrs at RT.
Immunohistochemistry (formalin/paraffin) 1-2ug/ml for 30 min at RT.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cells; tonsil or breast carcinoma
Cellular LocalizationCytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoV152
HostMouse
SourceHamster
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8453-10X.
PurityPurified by Protein G affinity chromatography.
ImmunogenHamster cyclin B1 protein
SpecificityRecognizes hamster Cyclin B1 at 62kD. Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

This product is offered by Biomol for research purposes only. Not for diagnostic purposes or human use. It may not be resold or used to manufacture commercial products without written approval of Biomol GmbH.

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