DNMT1 direct activity assay kit

Art Nr.: BPS-52050L, BPS
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DNMT1 Chemiluminescent Assay Kit

Catalog #: BPS-52050L
Size: 96 reactions
The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Supplied As:
Kit comes in a convenient format, with a 96-well plate precoated with DNMT substrate, the antibody against 5-methylcytosine, the secondary HRP-labeled antibody, S-adenosylmethionine, DNMT1 assay buffer, and purified DNMT1 for 100 enzyme reactions.
DMSO >1%, strong acids or bases, ionic detergents, high salt
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Useful for studying enzyme kinetics and HTS applications.
Svedruzic, Z.M. Curr Med Chem. 2008; 15(1) :92-106.
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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This product is offered by Biomol for research purposes only. Not for diagnostic purposes or human use. It may not be resold or used to manufacture commercial products without written approval of Biomol GmbH.