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Anti-AKT (PKB, rac)

Art Nr.: 600-601-401, Rockland
Biomol GmbH
Waidmannstr. 35
22769 HAMBURG
info@biomol.de
www.biomol.de
Tel:+49-40 - 85 32 600
0800 - 24 66 651 free call
Fax: +49-40 - 85 32 60-22
0800 - 24 66 652 free call

AKT Antibody Properties



Description Anti-AKT (SHEEP) Antibody - 600-601-401

Host Sheep

Target Species Human

Known Cross Reactivity human

Clonality Polyclonal

Application ELISA : 1:5,000 - 1:20,000
IF Microscopy : user optimized
FlowCytometry : User Optimized
Western Blot : 1:500 - 1:2,000
Immunochemistry: user optimized
Other Dilution: User Optimized

Physical State Liquid (sterile filtered)

Shipping condition Dry Ice

Format IgG

Label Unconjugated

Concentration 0.9 mg/mL by UV absorbance at 280 nm

Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative 0.1% (w/v) Sodium Azide

Stabilizer None

GeneName AKT1  

AKT Antibody Description

Background

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Immunogen

Anti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein.  A residue of cysteine was added to facilitate coupling.

Immunogen Type

Peptide

Storage Condition

Store Anti-AKT at -20 °C prior to opening. Aliquot antibody and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This Anti-AKT affinity purified antibody is suitable for ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user for IF and IHC.  Expect a band approximately 55-60 kDa in size corresponding to AKT1 by western blotting in the appropriate cell lysate or extract. Cell Signaling, Neuroscience, Signal Transduction research.

Purity/Specifity

This antibody is directed against AKT and reacts with the AKT from human tissues.  Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses.  J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Chu,S., Li,L., Singh,H. and Bhatia,R. (2007) BCR-tyrosine 177 plays an essential role in Ras and Akt activation and in human hematopoietic progenitor transformation in chronic myelogenous leukemia.  Cancer Res. 67 (14), 7045-7053.

Specific Reference

This product is offered by Biomol for research purposes only. Not for diagnostic purposes or human use. It may not be resold or used to manufacture commercial products without written approval of Biomol GmbH.

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