Fluorogenic SIRT1 (Sir2) Assay Kit / BPS Bioquest Product-Datasheet BPS-50081

Fluorogenic SIRT1 (Sirtuin1) Assay KitCatalog #: BPS-50081

Size: 96 reactions

DESCRIPTION: The Fluorogenic SIRT1 Assay Kit is a complete assay systemdesigned to measure Sirtuin 1 (SIRT1) activity for screening and profilingapplications. It comes in a convenient 96-well format, with all the reagentsnecessary for 100 fluorescent SIRT1 (Sir2) activity measurements. In addition,the kit includes purified SIRT1 enzyme and a SIRT inhibitor, Nicotinamide, foruse as a positive and negative control, respectively. The Fluorogenic SIRT1Assay Kit is based on a unique fluorogenic substrate and developer combination.This assay method eliminates dealing with the radioactivity, extraction, andchromatography aspects of traditional assays. Using this kit, only two simplesteps on a microtiter plate are needed to analyze the SIRT1 activity level. First,the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylatedlysine side chain, is incubated with purified SIRT1 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and screening smallmolecular inhibitors for drug discovery and HTS applications.

REFERENCES:1. A. Ito et al. (2001) EMBO J. 20 1331.2. N.A. Barlev et al. (2001) Mol. Cell 8 1243.3. A. Ito et al. (2002) EMBO J. 21 6236.