BRD3 (BD1) Inhibitor Screening Assay Kit / BPS Bioscience Product-Datasheet BPS-32513

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BRD3 (BD1) Inhibitor Screening Assay Kit

BRD3 (BD1) Inhibitor Screening Assay KitCatalog # 32513

Size: 384 reactions

DESCRIPTION: The BRD3 (BD1) Inhibitor Screening Assay Kit is designed to measurethe inhibition of BRD3 bromodomain 1 (BD1) binding to its substrate. The BRD3 (BD1)Inhibitor Screening Assay Kit comes in a convenient AlphaLISA® format, with biotinylatedhistone peptide substrate, assay buffer, detection buffer and purified, GST-tagged BRD3 BD1 toperform a total of 384 enzyme reactions. The key to the BRD3 (BD1) Inhibitor ScreeningAssay Kit is the highly specific binding of the BRD3 bromodomain to the acetylatedhistone substrate. With this kit, only three simple steps on a microtiter plate are required.First, a sample containing BRD3 bromodomain 1 and an inhibitor of choice is incubated with thebiotinylated substrate for thirty minutes. Next, acceptor beads are added, then donorbeads, followed by reading the Alpha-counts.

COMPONENTS:

BRD3 BD1 Components

APPLICATIONS: Useful for the study of bromodomain binding assays, screeninginhibitors, and selectivity profiling.

STABILITY: At least one year from date of receipt when stored as directed.

REFERENCE: Muller, S., Filippakopoulos, P., Knapp, S., Expert Rev. Mol. Med. 2011 Sep13;13:e29.

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