BRD3 (BD1) Inhibitor Screening Assay Kit

Art Nr.: BPS-32513, BPS
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BRD3 (BD1) Inhibitor Screening Assay Kit

Catalog #: BPS-32513
Size: 384 reactions
495
Description:
The BRD3 (BD1) Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD3 bromodomain 1 (BD1) from binding to its substrate. The key to the BRD3 (BD1) Inhibitor Screening Assay Kit is the highly specific binding of the BRD3 bromodomain 1 to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD3 bromodomain 1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Synonym(s):
BRD3, RING3L, Bromodomain
Supplied As:
Kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified, GST-tagged BRD3 BD1 to perform a total of 384 enzyme reactions.
Contraindications:

DMSO above 0.5%. Only limited amounts of DMSO can be included, as it has been shown to disrupt BRD-ligand interaction. Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.

Format:
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
Reference(s):
Muller S, Filippakopoulos P, Knapp S. Expert Rev Mol Med. 2011 Sep 13;13:e29.