Amplite(TM) Luciferase Reporter Gene Assay Kit *Bright Glow*

Art Nr.: ABD-12519, AAT Bioquest
Biomol GmbH
Waidmannstr. 35
22769 HAMBURG
info@biomol.de
www.biomol.de
Tel:+49-40 - 85 32 600
0800 - 24 66 651 free call
Fax: +49-40 - 85 32 60-22
0800 - 24 66 652 free call
Cat # ABD-12519 Storage F/D/L (see storage codes below)
Unit Size 10 plates Price EUR 395
Ex (nm) 328 Em (nm) 533
MW Solvent
Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The advantages of a luciferase assay are the high sensitivity, the absence of luciferase activity inside most of the cell types, the wide dynamic range, rapidity and low cost. The most versatile and common reporter gene is the luciferase of the North American firefly Photinus pyralis. The protein requires no posttranslational modification for enzyme activity. It is not even toxic in high concentration (in vivo) and can be used in pro- and eukaryotic cells. The firefly luciferase catalyzes the bioluminescent oxidation of luciferin in the presence of ATP, magnesium and oxygen. This Amplite™ Luciferase Reporter Gene Assay Kit uses a proprietary luminogenic formulation to quantify luciferase activity in live cells and cell extracts. Our formulation generates a luminescent product that gives strong luminescence upon interaction with luciferase. The kit provides all the essential components with our optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments. It has extremely high sensitivity, and can be used for the assays that require demanding sensitivity.

Storage Codes:

  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 °C)
  R = Refrigerated (2-4 °C)
  RT = Room temperature (4-50 °C)

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