The Western blot (alternatively, protein immunoblot) is a widely used analytical technique used to detect specific proteins in the given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein (ref.).
Rockland Immunochemicals offers several western blotting kits. Download this two paged pdf flyer describing Rockland Western Blotting Kits.
Rockland Immunochemicals’ FemtoMax Chemiluminescent Western Blot Kit combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of Rockland´s luminol substrate. The Chemiluminescent Western Blot Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored at +4°C. Because of the extremely high sensitivity of our FemtoMax Chemiluminescent Substrate Kit for Western Blotting, primary and secondary antibodies can be used at greater dilutions. If you currently dilute your primary antibody 1:1,000 using an ECL substrate equivalent, then dilute your antibody 1:5,000 using the FemtoMax substrate.
Rockland Immunochemicals recently announced a new Green Fluorescent Protein (GFP) Western Blot Kit. GFP is routinely used as a reporter of expression. GFP is an ideal biosensor and numerous cell lines have been created that express GFP. Rockland’s GFP Western Blot Kit (catalogue number KCA215) was produced by optimizing Rockland’s Anti-GFP Antibody (catalogue number 600-301-215). Rockland’s GFP western blot kit has been validated for use in Western Blotting. The Anti-GFP antibody is also functional in ELISA, Immunohistochemistry and immunofluorescent microscopy. GFP Antibodies and Anti-GFP Conjugates are a simple choice for your GFP detection needs. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Rockland produces other epitope tag antibodies including RFP Antibodies, 6X His Antibodies and Anti-GST Antibodies....
Molecular Weight Markers for use in western blotting: