PCR Arrays are the most reliable tools for analyzing the expression of a focused panel of genes. Each 96-well or 384-well plate includes SYBR Green-optimized primer assays for a thoroughly researched panel of relevant, pathway- or disease-focused genes. PCR Arrays can also be customized to contain a panel of genes tailored to your specific research interests. Our high-quality primer design and master mix formulation enable the PCR Array to amplify 96 or 384 different gene-specific products simultaneously under uniform cycling conditions. This combination provides the PCR Array with the specificity and the high amplification efficiencies required for accurate real-time SYBR Green results. The simplicity of the PCR Arrays makes them accessible for routine use in every research laboratory.
New developments in PCR Array technology enable optimal performance of RT² Profiler PCR Arrays using RNA prepared from regular samples (0.1 - 5 ug RNA), FFPE samples, and small samples (1 - 100 ng RNA). See the PCR Array Protocol Guide to learn more.
Why PCR Arrays?
Performance and Applications of PCR Arrays
Application Examples
The PCR Arrays have been used for cancer, immunology, stem cells, toxicology, and many other areas of biological and medical research. To view application examples for cancer and toxicology research, click [ here ]. To see a list of publications citing the use of the PCR Array, click [ here ].
How PCR Arrays Work
Simple Workflow
Simply mix your cDNA template with the appropriate ready-to-use PCR master mix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. PCR Arrays are compatible with all ABI, Bio-Rad, Stratagene and Roche instrumentation. Find the correct setup instructions and protocol files [ here ].
Well Designed Layout and Controls
The PCR Arrays are available in both 96- and 384-well plates and are used to monitor the expression of 84 or 370 genes related to a disease state or pathway plus five housekeeping genes. Controls are also included on each array for genomic DNA contamination, RNA quality, and general PCR performance. Click [ here ] to learn how the control features work.
Easy-to-Use Data Analysis
Download an easy-to-use Excel-based data analysis template [ here ]. Data analysis is based on the ΔΔCt method with normalization of the raw data to either housekeeping genes or an external RNA control.
You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Services.
PCR Array Product Support Links
Technical Support
| Working Protocol | Required Reagents and Instruments |
| Overview of PCR Arrays | Instrument Setup Protocol |
| Performance Data | PCR Array Layout |
| User Manual | Controls |
| Data Analysis |
Presentations and Publications
| View a Slide Presentation | Publications |
| Attend a Live Web Seminar |
Purchase Support
| Complete List of PCR Arrays | Instrument-Specific qPCR Master Mix |
| Other RT-PCR Accessories | Buyer\'s Guide (Information for users of Real Time PCR Cyclers from Applied Biosystems (ABI), Bio-Rad, Stratagene, Roche, Eppendorf, MJ Research) |



