HERSTELLER

Immunoprecipitation


Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure (ref.).

Agarose/Sepharose Products


Agarose/Sepharose Conjugated Protein G/Protein A

Agarose Conjugated Secondary Antibodies


Avoid the use of Protein A or Protein G by using secondary antibodies directly coupled to agarose.

Agarose Conjugated Epitope Tag Antibodies



Epitope Tag Antibody Agarose Conjugate

 Recognition Site

 6xHis Epitope Tag Antibody Agarose Conjugate  HHHHHH
 AU1 Epitope Tag Antibody Agarose Conjugate  DTYRYI
 AU5 Epitope Tag Antibody Agarose Conjugate  TDFYLK
 c-myc Epitope Tag Antibody Agarose Conjugate  EQKLISEEDL
 ECS Epitope Tag Antibody Agarose Conjugate  DDDDK
 E-Tag Epitope Tag Antibody Agarose Conjugate  GAPVPYPDPLEPR
 Glu-Glu Epitope Tag Antibody Agarose Conjugate  EYMPME
 GST Antibody Agarose Conjugate  Mature Protein
 HA Epitope Tag Antibody Agarose Conjugate  YPYDVPDYA
 HSV Epitope Tag Antibody Agarose Conjugate  SQPELAPEDPED
 KT3 Epitope Tag Antibody Agarose Conjugate  KPPTPPPEPET
 S-Tag Epitope Tag Antibody Agarose Conjugate  KETAAAKFERQHMDS
 T7 Epitope Tag Antibody Agarose Conjugate  MASMTGGQQMG
 V5 Epitope Tag Antibody Agarose Conjugate  GKPIPNPLLGLDST
 VSV-G Epitope Tag Antibody Agarose Conjugate  YTDIEMNRLGK


ECS (DDDDK) tagged proteins are also known as flag-tagged proteins. FLAG™ and ANTI-FLAG™ are a registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.



Antibodies working in immunoprecipitation

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Immunoprecipitation Protocols

Immunoprecipitation Kit

Immunoprecipitation followed by Western Blotting


TrueBlot for Optimum WB after IP

If you want to use a rabbit antibody for immunoprecipiation as well as detection by western blotting, we recommend ReliaBLOT Immunoprecipitation/Western Blot Reagents.

Cell Lysis Buffer

Protease Inhibitors


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