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Immunoprecipitation

Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure (ref.).

 

Agarose/Sepharose Products

Agarose/Sepharose Conjugated Protein G/Protein A

 

Agarose Conjugated Secondary Antibodies

Avoid the use of Protein A or Protein G by using secondary antibodies directly coupled to agarose.

 

Agarose Conjugated Epitope Tag Antibodies

Epitope Tag Antibody Agarose Conjugate

Recognition Site

6xHis Epitope Tag Antibody Agarose Conjugate HHHHHH
AU1 Epitope Tag Antibody Agarose Conjugate DTYRYI
AU5 Epitope Tag Antibody Agarose Conjugate TDFYLK
c-myc Epitope Tag Antibody Agarose Conjugate EQKLISEEDL
ECS Epitope Tag Antibody Agarose Conjugate DDDDK
E-Tag Epitope Tag Antibody Agarose Conjugate GAPVPYPDPLEPR
Glu-Glu Epitope Tag Antibody Agarose Conjugate EYMPME
GST Antibody Agarose Conjugate Mature Protein
HA Epitope Tag Antibody Agarose Conjugate YPYDVPDYA
HSV Epitope Tag Antibody Agarose Conjugate SQPELAPEDPED
KT3 Epitope Tag Antibody Agarose Conjugate KPPTPPPEPET
S-Tag Epitope Tag Antibody Agarose Conjugate KETAAAKFERQHMDS
T7 Epitope Tag Antibody Agarose Conjugate MASMTGGQQMG
V5 Epitope Tag Antibody Agarose Conjugate GKPIPNPLLGLDST
VSV-G Epitope Tag Antibody Agarose Conjugate YTDIEMNRLGK

ECS (DDDDK) tagged proteins are also known as flag-tagged proteins. FLAG™ and ANTI-FLAG™ are a registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.


Antibodies working in immunoprecipitation

 

Immunoprecipitation Protocols

 

Immunoprecipitation Kit

 

Immunoprecipitation followed by Western Blotting

If you want to use a rabbit antibofy for immunoprecipiation as well as detection by western blotting, we recommend ReliaBLOT Immunoprecipitation/Western Blot Reagents.

 

Cell Lysis Buffer

 

Protease Inhibitors


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