HERSTELLER

Immunohistochemistry


Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

Rockland Immunohistochemistry Kits

Bethyl Immunohistochemistry Kit

Spring Bioscience IHC Detection

REVEAL™ Biotin-Free System vs. conventional polymer systems for detection in immunohistochemistry.

Antibody Labeling Kits

Use directly labeled primary antibodies for better signal-to-noise ratio. With Lightning-Link antibody labelling kits it takes only 30 seconds hands-on-time to label the antibody of your choice.

Counterstain and Substrate Compability Table


 

 

Blue Counterstain

Red Counterstain

 

Substrate (Catalog No.),
color

Mayer\'s Hematoxylin Solution (S-99025),
intensive blue
Nuclear Blue Solution (S-99035),
light blue

Nuclear Fast Red Solution (S-99031),
intensive red
Nuclear Red Solution (S-99033),
light red

AP-Detection

AP-Red Solution Set ISH (S-99061),
red

excellent contrast

color incompatibility

AP-Red Solution Set IHC (S-99065),
red

excellent contrast

color incompatibility

NBT/BCIP Solution (S-99064),
dark blue/violet

color incompatibility

excellent contrast

HRP-Detection

AEC Solution ISH* (S-99010),
red

excellent contrast

color incompatibility

AEC Solution IHC* (S-99012),
red

excellent contrast

color incompatibility

DAB Solution Set (S-99014),
brown

excellent contrast

fair contrast

HRP-Green Solution Set (S-99056),
green

good contrast

excellent contrast


*The reaction product is soluble in alcohol. Thus, aqueous counterstains and mounting media must be used.



Antibodies working in immunohistochemistry

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Immunohistochemistry Protocols

Tissue Fixation

Antigen Retrieval

IHC Substrates

Counterstains

Secondary Antibodies for Immunohistochemistry


Primary Antibodies for IHC


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