Immunohistochemistry
Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.
Rockland Immunohistochemistry Kits
Bethyl Immunohistochemistry Kit
Spring Bioscience IHC Detection
REVEAL™ Biotin-Free System vs. conventional polymer systems for detection in immunohistochemistry.
Antibody Labeling Kits
Use directly labeled primary antibodies for better signal-to-noise ratio. With
Lightning-Link antibody labelling kits it takes only
30 seconds hands-on-time to label the antibody of your choice.
Counterstain and Substrate Compability Table
| | Blue Counterstain | Red Counterstain |
| Substrate (Catalog No.),
color | Mayer\'s Hematoxylin Solution (S-99025),
intensive blue
Nuclear Blue Solution (S-99035),
light blue | Nuclear Fast Red Solution (S-99031),
intensive red
Nuclear Red Solution (S-99033),
light red |
AP-Detection | AP-Red Solution Set ISH (S-99061),
red | excellent contrast | color incompatibility |
AP-Red Solution Set IHC (S-99065),
red | excellent contrast | color incompatibility |
NBT/BCIP Solution (S-99064),
dark blue/violet | color incompatibility | excellent contrast |
HRP-Detection | AEC Solution ISH* (S-99010),
red | excellent contrast | color incompatibility |
AEC Solution IHC* (S-99012),
red | excellent contrast | color incompatibility |
DAB Solution Set (S-99014),
brown | excellent contrast | fair contrast |
HRP-Green Solution Set (S-99056),
green | good contrast | excellent contrast |
*The reaction product is soluble in alcohol. Thus, aqueous counterstains and mounting media must be used.
Antibodies working in immunohistochemistry
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Immunohistochemistry Protocols
Tissue Fixation
Antigen Retrieval
IHC Substrates
Counterstains
Secondary Antibodies for Immunohistochemistry
Primary Antibodies for IHC
