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Immunohistochemistry

Immunohistochemistry or IHC refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

 

Rockland Immunohistochemistry Kits

 

Bethyl Immunohistochemistry Kit

 

Antibody Labeling Kits

Use directly labeled primary antibodies for better signal-to-noise ratio. With Lightning-Link antibody labelling kits it takes only 30 seconds hands-on-time to label the antibody of your choice.

 

Counterstain and Substrate Compability Table

   

Blue Counterstain

Red Counterstain

 

Substrate (Catalog No.),
color

Mayers Hematoxylin Solution (S-99025),
intensive blue
Nuclear Blue Solution (S-99035),
light blue

Nuclear Fast Red Solution (S-99031),
intensive red
Nuclear Red Solution (S-99033),
light red

AP-Detection

AP-Red Solution Set ISH (S-99061),
red

excellent contrast

color incompatibility

AP-Red Solution Set IHC (S-99065),
red

excellent contrast

color incompatibility

NBT/BCIP Solution (S-99064),
dark blue/violet

color incompatibility

excellent contrast

HRP-Detection

AEC Solution ISH* (S-99010),
red

excellent contrast

color incompatibility

AEC Solution IHC* (S-99012),
red

excellent contrast

color incompatibility

DAB Solution Set (S-99014),
brown

excellent contrast

fair contrast

HRP-Green Solution Set (S-99056),
green

good contrast

excellent contrast

*The reaction product is soluble in alcohol. Thus, aqueous counterstains and mounting media must be used.


Antibodies working in immunohistochemistry

 

Immunohistochemistry Protocols

 

Antigen Retrieval

 

IHC Substrates

 

Counterstains

 

Polymer Antibody for Immunohistochemistry

 

Secondary Antibodies for Immunohistochemistry

 

Primary Antibodies for IHC


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