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Epigenetics

Histone ModificationThe term epigenetics refers to heritable changes in phenotype (appearance) or gene expression caused by mechanisms other than changes in the underlying DNA sequence. This change is accomplished by modification of chromatin, eg. g. DNA (methylation of cytosine) or acetylation or methylation of histones.

Biomol offers several tools for the researcher, which are presented here.

 

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation (ChIP for short) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This technique gives a picture of the protein-DNA interactions that occur inside the nucleus of living cells or tissues.

 

ChIP KitThe principle of this assay is that DNA-binding proteins (including transcription factors and (modified) histones) in living cells can be cross-linked to the DNA that they are binding. By using an antibody that is specific to a putative DNA binding protein, one can immunoprecipitate the protein-DNA complex out of cellular lysates. Following crosslinking, the cells are lysed and the DNA is broken into pieces 0.2 kB (for classic PCR detection) to 1 kb (for qPCR detection) in length by sonication. At this point the immunoprecipitation is performed resulting in the purification of protein-DNA complexes. The purified protein-DNA complexes are then heated to reverse the formaldehyde cross-linking of the protein and DNA complexes, allowing the DNA to be separated from the proteins. The identity and quantity of the DNA fragments isolated can then be determined by PCR or - more precisely - by qPCR. Biomols offers a ChIP kit for PCR detection.



Imgenex QuikChIP™ Kits for classical PCR detection

Imgenex offesr a range of ChIP products (more Info):

ChIP qualified Antibodies

In order to immunoprecipitate chromatin you need antibodies capable to do so: Imgenex offers several ones as well as Bethyl and Rockland Immunochemicals. For a complete listing please click [here].

 

Histone ELISA Kits and Antibodies

If you are interested in determination of the amount of a specific histone modification, you can detect it with specific antibodies via western blotting, immunostaining (IHC, ICC, FC or IP) or by ELISA. There are antibodies specific phosphorylated, acetylated or methylated histones.

 

DNA Methylation

Chromatin remodeling, an epigenetic way of gene expression regulation, is achieved besides post translational modification of histone proteins by the addition of methyl groups to the DNA, at CpG sites, to convert cytosine to 5-methylcytosine. This is catalyzed by DNA methyltransferases (DNMT). Biomol offers antibodies to DNA Methyltransferase and chromatin remodeling proteins.

 

Chromatin Remodelling Protein Antibodies

Bethyl produces antibodies against chromatin remodelling proteins:

 

DNMT Antibodies

Biomol offers several antibodies to DNA methyltransferases:

See also: IMGENEX HDAC and Dnmt Antibodies. Histone Methylation: HMT-Demethylase Pairs


Deacetylases: HDACs, Sirtuins

HDACs and Sirtuins: Assay kits, enzymes, and substrates from BPS Bioscience.

HDAC Assay Kits

 Cat #   Size   Product Name 
 BPS-50033   100 assays   Fluorogenic HDAC Assay Kit 
 BPS-50034   100 assays   Fluorogenic HDAC Assay Kit (green) 
 BPS-50041   100 assays   Fluorogenic HDAC Class 2a Assay Kit 
 BPS-50068   100 assays   Fluorogenic HDAC8 Assay Kit 
 BPS-50081   100 assays   Fluorogenic SIRT1 (Sir2) Assay Kit 
 BPS-50082   100 assays   Fluorogenic SIRT2 Assay Kit 
 BPS-50083   100 assays   Fluorogenic SIRT3 Assay Kit 
 BPS-50031   20 mL   HDAC Assay Buffer 
 BPS-50030   6 mL   HDAC Assay Developer 
 BPS-50089   6 mL   SIRT Assay Developer 
 BPS-50086   96 reactions   Sirtuin 6 (SIRT6) Chemiluminescent Assay Kit 

 

Deacetylase Enzymes

 Cat #   Size   Product Name 
 BPS-50051   50 µg   HDAC1 
 BPS-50052   50 µg   HDAC2 
 BPS-50002   50 µg   HDAC2 (C-His) 
 BPS-50003   50 µg   HDAC3/NcoR2 
 BPS-50004   2 µg   HDAC4 
 BPS-50005   5 µg   HDAC5 
 BPS-50045   5 µg   HDAC5 full length 
 BPS-50006   50 µg   HDAC6 
 BPS-50007   2 µg   HDAC7 
 BPS-50008   50 µg   HDAC8 
 BPS-50009   5 µg   HDAC9 
 BPS-50010   50 µg   HDAC10 (N-GST) 
 BPS-50060   50 µg   HDAC10 (N-Flag) 
 BPS-50011   50 µg   HDAC11 
 BPS-50020   50 µg   NcoR2/SMRT 
 BPS-50063   20 µg   pf-HDAC 1  
 BPS-50012   100 µg   Sirtuin 1 
 BPS-50013   100 µg   Sirtuin 2 
 BPS-50014   100 µg   Sirtuin 3 
 BPS-50015   100 µg   Sirtuin 4 
 BPS-50016   100 µg   Sirtuin 5 
 BPS-50017   100 µg   Sirtuin 6 
 BPS-50018   100 µg   Sirtuin 7 

 

Deacetylase Substrates

 Cat #   Size   Product Name 
 BPS-50035   250 nmol   Fluorogenic Deacetylated Substrate Standard 
 BPS-50040   250 nmol   Fluorogenic HDAC class 2a Substrate 1 
 BPS-50042   250 nmol   Fluorogenic HDAC class 2a substrate 2 (Green fluorescence) 
 BPS-50032   250 nmol   Fluorogenic HDAC Substrate 1 
 BPS-50038   250 nmol   Fluorogenic HDAC Substrate 2 (Green fluorescence) 
 BPS-50037   250 nmol   Fluorogenic HDAC Substrate 3 

 

Methyltransferases

Methyltransferases for epigenetic and other research from BPS Bioscience: assay kits, enzymes, and substrates.

Methyltransferase Assay Kits

 Cat #   Size   Product Name 
 BPS-52200   30 mL   4x DNMT Assay Buffer 1 
 BPS-52201   30 mL   4x DNMT Assay Buffer 2 
 BPS-52160   30 mL   4x HMT Assay Buffer 1 
 BPS-52170   30 mL   4x HMT Assay Buffer 2 
 BPS-52180   30 mL   4x HMT Assay Buffer 3 
 BPS-52190   30 mL   4x HMT Assay Buffer 4 
 BPS-52030   1 mL   Adenosyl Homocysteine Antibody 
 BPS-52035   96 reactions   DNMT Universal Assay Kit 
 BPS-52050L   100 reactions   DNMT1 Assay Kit 
 BPS-52033   100 reactions   DNMT3A Assay Kit 
 BPS-52034   100 reactions   DNMT3B Assay Kit 
 BPS-52009L   100 reactions   EZH2 Chemiluminescent Assay Kit 
 BPS-52012   20 µL @ 12.5 µM   Fluorescein-labeled S-adenosyl homocysteine 
 BPS-52001L   100 reactions   G9a Chemiluminescent Assay Kit 
 BPS-52051   384 reactions   G9a Homogenous Assay Kit 
 BPS-52078   96 reactions    Histone H3(K4) Universal Methyltransferase Assay Kit 
 BPS-52071   96 reactions    Histone H3(K9) Universal Methyltransferase Assay Kit 
 BPS-52074   96 reactions    Histone H3(R3) Universal Methyltransferase Assay Kit 
 BPS-52100   50 mL     Methyltransferase Blocking Buffer 
 BPS-52008L   100 reactions   MLL Chemiluminescent Assay Kit 
 BPS-52004L   100 reactions   PRMT1 Chemiluminescent Assay Kit 
 BPS-52005L   100 reactions   PRMT3 Chemiluminescent Assay Kit 
 BPS-52002L   100 reactions   PRMT5 Chemiluminescent Assay Kit 
 BPS-52003L   100 reactions   Set7/9 Chemiluminescent Assay Kit 
 BPS-52006L   100 reactions   SUV39H1 Chemiluminescent Assay Kit 
 BPS-52007L   100 reactions   SUV39H2 Chemiluminescent Assay Kit 

 

Methyltransferase Enzymes

 Cat #   Size   Product Name 
 BPS-51101   10 µg   DNMT1 
 BPS-51102   10 µg   DNMT2 
 BPS-51103   10 µg   DNMT3a 
 BPS-51109   10 µg   DNMT3B/DNMT3L 
 BPS-51105   10 µg   DNMT3B (mouse) 
 BPS-51005   50 µg   Dot1L 
 BPS-51009   5 µg   Dot1L (full length) 
 BPS-50280   50 µg   EED 
 BPS-51006   50 µg   EZH1/EED/SUZ12 
 BPS-51007   50 µg   EZH1/EED/SUZ12/RbAp48/AEBP2 
 BPS-51002   20 µg   EZH2/EED inactive 
 BPS-51003   50 µg   EZH2/EED/SUZ12 
 BPS-51099   50 µg   EZH2/EED/SUZ12/RbAp48 
 BPS-51004   50 µg   EZH2/EED/SUZ12/RbAp48/AEBP2 
 BPS-51000   50 µg   G9a (E.coli) 
 BPS-51001   20 µg   G9a (insect) 
 BPS-51018   20 µg   Metnase (SETMAR) 
 BPS-51020   50 µg   MLL 
 BPS-51026   50 µg   NSD2 (catalytic domain) 
 BPS-51025   20 µg   NSD2(782-end)/ReBPII 
 BPS-51030   200 µg   PRDM2(RIZ1) 
 BPS-51040   50 µg   PRMT1 (E.coli) 
 BPS-51041   20 µg   PRMT1 (Sf9) 
 BPS-51043   50 µg   PRMT3 
 BPS-51047   20 µg   PRMT4/CARM1 (HEK293) 
 BPS-51045   20 µg   PRMT5 (HEK293) 
 BPS-51048   10 µg   PRMT5 (Sf9) 
 BPS-51046   20 µg   PRMT6 
 BPS-51051   20 µg   PRMT7 
 BPS-51012   100 µg   Set2(HYPB) 
 BPS-51010   100 µg   Set7/Set9 
 BPS-51008   50 µg   Set8 
 BPS-51014   20 µg   SMYD2 
 BPS-51015   20 µg   SMYD3 (35-end) 
 BPS-51016   20 µg   SMYD3 (Full Length) 
 BPS-51070   50 µg   SUV39H1 (82-end) 
 BPS-51071   5 µg   SUV39H1 (Full Length) 
 BPS-51080   50 µg   SUV39H2 
 BPS-51090   50 µg   SUV4-20h1 
 BPS-51060   50 µg   SUV4-20h2 

 

Methyltransferase Substrates

 Cat #   Size   Product Name 
 BPS-52011   80 nmole   Biotinylated histone H3 peptide (1-21) 
 BPS-52017   80 nmole   Biotinylated histone H3 peptide (21-44) 
 BPS-52018   80 nmole   Biotinylated histone H4 peptide (1-21) 
 BPS-51200   500 µL   di-methylated R3_H4 peptide substrate, biotinylated 
 BPS-52013   500 µg   Histone H3 (2-58) 
 BPS-52010   160 nmole   Histone H3 peptide (1-21) 
 BPS-52016   160 nmole   Histone H3 peptide (21-44) 
 BPS-52014   160 nmole   Histone H4 peptide (1-21) 
 BPS-52015   50 reactions   Nucleosomes 

 

Demethylases

Demethylases for epigenetic and other research from BPS Bioscience: assay kits, enzymes, and substrates.

Demethylase Assay Kits

 Cat #   Size   Product Name 
 BPS-50413   384 reactions   JMJD2A Homogenous Assay Kit 
 BPS-50414   384 reactions   JMJD2B Homogenous Assay Kit 
 BPS-50405   100 reactions   JMJD2C Luminescent Assay Kit 
 BPS-50415   384 reactions   JMJD2C Homogenous Assay Kit 
 BPS-50417   384 reactions   JMJD2E Homogenous Assay Kit 
 BPS-50109   96 reactions   LSD1 Chemiluminescent Assay Kit 
 BPS-50107   400 reactions   LSD1 Fluorescent Assay Kit (384 well) 
 BPS-50106   100 reactions   LSD1 Fluorescent Assay Kit (96 well) 
 BPS-50108   384 reactions   LSD1 Homogeneous Assay Kit 

 

Demethylase Enzymes

 Cat #   Size   Product Name 
 BPS-50120   20 µg   FBXL10 (KDM2B, JHDM1B)
 BPS-50102   20 µg   FBXL11 (KDM2A)
 BPS-50110   20 µg   JARID1A (KDM5A, RBBP2)
 BPS-50103   20 µg   JMJD2A (KDM4A)
 BPS-50104   20 µg   JMJD2B (KDM4B)
 BPS-50105   20 µg   JMJD2C (GASC1KDM4C
 BPS-50118   20 µg   JMJD2E (KDM4DL)
 BPS-50115   20 µg   JMJD3 (KDM6B
 BPS-50140   20 µg   JMJD6 
 BPS-50100   50 µg   LSD1 (KDM1) (AOF2)
 BPS-50122   20 µg   PLU-1 (JARID1BKDM5B
 BPS-50116   20 µg   UTX (KDM6A

 

Demethylase Substrates

 Cat #   Size   Product Name 
 BPS-50351   500 µL   di-methylated K9_H3 peptide substrate, biotinylated 
 BPS-50101   500 µL   LSD1 substrate (di-methylated K4_H3 peptide substrate)  
 BPS-50350   500 µL   tri-methylated K9_H3 peptide substrate, biotinylated 

 

Poly ADP Ribose Polymerases (PARP)

Poly ADP Ribose Polymerases (PARP) for DNA repair, apoptosis and other research from BPS Bioscience: assay kits, enzymes, and substrates.

 

Poly ADP Ribose Polymerase (PARP) Assay Kits

 Cat #   Size   Product Name 
 BPS-80551    100 reactions   PARP1 Assay Kit 
 BPS-80552    100 reactions   PARP2 Assay Kit 
 BPS-80553    100 reactions   PARP3 Assay Kit 
 BPS-80556    32 reactions   PARP6 Assay Kit 
 BPS-80557    96 reactions   PARP7 Assay Kit 
 BPS-80561    96 reactions   PARP11 Assay Kit 
 BPS-80554    96 reactions   Tankyrase-1 Chemiluminescent Assay Kit 
 BPS-80555    96 reactions   Tankyrase-2 Chemiluminescent Assay Kit 

 

Poly ADP Ribose Polymerase (PARP) Enzymes

 Cat #   Size   Product Name 
 BPS-80501   10 µg   PARP1 
 BPS-80502   10 µg   PARP2 
 BPS-80503   10 µg   PARP3 
 BPS-80504   10 µg   TNKS1 (PARP5A) 
 BPS-80505   10 µg   TNKS2 (PARP5B/C) 
 BPS-80506   10 µg   PARP6 
 BPS-80507   10 µg   PARP7 
 BPS-80509   10 µg   PARP9 
 BPS-80511   10 µg   PARP11 
 BPS-80512   10 µg   PARP12 

 

Poly ADP Ribose Polymerase (PARP) Substrates

 Cat #   Size   Product Name 
 BPS-80605   500 µL   Activated DNA 
 BPS-52021   1 mg   Histone H2A (full length) 
 BPS-52025   100 µg   Histone H2A (full length) 
 BPS-52022   1 mg   Histone H2B (full length) 
 BPS-52026   100 µg   Histone H2B (full length) 
 BPS-52023   1 mg   Histone H3.1 (full length) 
 BPS-52027   100 µg   Histone H3.1 (full length) 
 BPS-52024   1 mg   Histone H4 (full length) 
 BPS-52028   100 µg   Histone H4 (full length) 
 BPS-52029   1 mL   Histone Mixture 
 BPS-80610   100 µL   NAD+, Biotin-labeled 

Biomol offers also antibodies for the detection of poly ADP ribose polymerases (PARP).

Methyltransferases from Cayman Chemical

Ash2L (human recombinant) DNA Methyltransferase 3L (human recombinant) G9a (human recombinant) PRMT1 (human recombinant) SET7/9 (human recombinant) SET8 (human recombinant) SUV4-20H1 (human recombinant) SUV4-20H2 (human recombinant)

 

HDAC and Sirtuin Enzymes from BPS Bioscience

BPS Bioscience is the leading producer of HDAC and Sirtuins enzymes: HDAC1, HDAC2, HDAC3, HDAC4, HDAC5 (full length), HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, Sirt1, Sirt2, Sirt3, Sirt4, Sirt5, Sirt6, ... Biomol offer these enzymes as well as assay kits and substrates: Learn more ...

 

Methyltransferases from BPS Bioscience

Also available: methyltransferase enzymes, assay kits and substrates for epigenetic research.

 

Research Tools Order Info

and much more: List of all gene regulation research products

 

Epigenetics Guide

 

Cayman Chemical


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