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ELISA

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competetive ELISA, often called EIA - analogous to RIA. The latter technique is preferred for small analytes.

 

ELISA Solutions

 

ELISA Antibody Pairs

 

Antibodies Tested for Use in ELISA

 

ELISA Kits

 

Antibody Stabilizers

 

ELISA Blocking Solutions

 

Plate Coating Buffers

 

HRP-Conjugate Stabilizer Diluents

 

Assay Diluents

 

Sample Diluents


ELISA Protocols

 

ELISA Substrates

Classical Substrates:

 

AAT Bioquest Alkaline Phosphatase Substrate Kits:

 

AAT Bioquest Horseradish Peroxidase (HRP) Substrate Kits:

 

Wash Buffers

 

PBS


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