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Western Blot: Blocking Buffers, Molecular Weight Markers and Positive Control Lysates


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Rockland produces accessory reagents for protein electrophoresis and western blotting of superior quality.

Blocking Buffers


  • Bovine Serum Albumin (BSA) Fraction V (immunoglobulin & protease free) BSA-10 10 g
  • 30% Bovine Serum Albumin Solution BSA-30 500 mL
  • 10X PBS Fish Gel Concentrate (azide and mercury free) MB-066-0100 100 mL
  • Infrared Imaging Blocking Solution MB-070 500 mL
  • BLOTTO B501-0500 500 g

Molecular Weight Marker


This Protein molecular weight marker is a cocktail of seven purified proteins that range in size from 14 to 120 kDa and thus span a molecular weight range common to most proteins and protein subunits. The protein mixture has been formulated to yield well-defined bands after SDS-polyacrylamide gel electrophoresis and staining with Coomassie blue or other common staining techniques. The intensity of the 67 kDa bovine serum albumine band has been increased to serve as a reference. The product is supplied in 1X loading buffer and is ready-to-use.

Positive Control Lysates


Western blotting positive controls. Each vial contains 500 µg of protein in 500 µL (except the nuclear extract as noted). Lysate is in 1X SDS PAGE Sample Buffer. All lysates are tested by SDS-PAGE using 4-20% gradient gels using antibodies to key signaling components to confirm the presence of both high molecular and low molecular weight proteins.

Loading Control Antibodies



More Western Blotting Reagents


HRP Substrates


Sensitive and Supersensitive Chemiluminescent HRP Substrates for Western Blotting and Microwell Assays

Western Blotting Kits


High Sensitivity Chemiluminescnce Western Blotting Kits
MaxTag Immunoblotting Kits

Primary Antibodies for Western Blotting


Rockland Immunochemicals produces more than 1000 primary antibodies for western blotting.

Secondary Antibodies


Use the Secondary Antibody Search for IRDye700-, IRDye800-, DyLight680-, DyLight800-, AP- and HRP- conjugated secondary antibodies.

Overview on IRDye antibodies.

Secondary Antibody Search

 



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