ATTO Conjugated Antibodies

ATTO-fluorescent dyes are a new generation of patented fluorescent markers.
Rockland Immunochemicals conjugates a select group of secondary antibodies to a this new generation of patented fluorescent markers from ATTO-TEC including ATTO 425, ATTO 488, ATTO 532, ATTO 550 , ATTO 594, ATTO 647N and ATTO655. The antibodies are designed for antibody detection and multiplex, multi-color analysis. ATTO-TEC fluorochrome conjugates offer

strong absorption (high extinction coefficient),
high fluorescence quantum yield and
superior high photostability.

These conjugates are ideal for various immunofluorescence based assays including

immunofluorescence microscopy,
FLISA,
STED microscopy,
fluorescent western blotting,
time resolved spectroscopy and
FRET (Fluorescence Resonance Energy Transfer) applications as well as
single-molecule detection (SMD).

The secondary antibodies conjugated to Atto dyes are available in two packing sizes (100 µg and 500 µg).

Rockland Immunochemicals offers Atto-conjugated secondary antibodies directed against primary antibodies from:

ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown above are:
A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody.
B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red).
C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue).
D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

Download this overview table of Atto conjugated secondary antibodies (pdf with links to order informations).