Anti-phospho-4E Binding Protein 1 (EIF4EBP1, Eukaryotic Translation Initiation Factor 4E-binding Pro

Anti-phospho-4E Binding Protein 1 (EIF4EBP1, Eukaryotic Translation Initiation Factor 4E-binding Pro
Item number Size Datasheet Manual SDS Delivery time Quantity Price
0004-21Q.50 50 µg - -

3 - 19 business days*

425.00€
 
4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12kD acidic protein that compete with... more
Product information "Anti-phospho-4E Binding Protein 1 (EIF4EBP1, Eukaryotic Translation Initiation Factor 4E-binding Pro"
4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12kD acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E- BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 65. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA:1:20,000, Western Blot:1:500-1:1,000, Immunohistochemistry:1:50-1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: 0004-21Q

Properties

Application: ELISA, IHC, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Species reactivity: human, mouse, rat
Immunogen: Synthetic phosphopeptide corresponding to human 4E-BP1 around the phosphorylation site of serine 65.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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