Detection of Human Cells in Xenografts
You want to identify the human cells in a xenograft? You want to know which cells in the tissue are of human origin and which are not?
You need an antibody that stains human cells and that gives no signal in mouse or rat cells. It would be nice if the nuclei would be stained to make it easy to identify the cells in the tissue.
This kind of antibody is within easy reach: Anti-Human Nuclei 1590-NUC
from PhosphoSolution for immunohistochemistry and immunofluorescent staining of human cells in xenografts.
The antibody can be used in a 1:100 dilution with PFA or aceton fixed cells. For FFPE tissue the antibody works better at 1:20.
A figure showing staining of human cells with clone 235-1 in presence of murine cells and staining of all nuclei with DAPI
can be found here
Application References of Anti-Human Nuclei, clone 235-1
Significant clinical, neuropathological and behavioural recovery from acute spinal cord trauma by transplantation of a well-defined somatic stem cell
Schira J, Gasis M, Estrada V, Hendricks M, Schmitz C, Trapp T, Kruse F, Kögler G, Wernet P, Hartung HP, Müller HW (2011) Brain : a journal of neurology
Siomycin A targets brain tumor stem cells partially through a MELK-mediated pathway.
Nakano I, Joshi K, Visnyei K, Hu B, Watanabe M, Lam D, Wexler E, Saigusa K, Nakamura Y, Laks DR, Mischel PS, Viapiano M, Kornblum HI (2011) Neuro-oncology.13:622-34. Epub 2011 May 9.
Transplantation of umbilical cord mesenchymal stem cells alleviates lupus nephritis in MRL/lpr mice.
Gu Z, Akiyama K, Ma X, Zhang H, Feng X, Yao G, Hou Y, Lu L, Gilkeson GS, Silver RM, Zeng X, Shi S, Sun L (2010) Lupus.19:1502-14. Epub 2010 Jul 20.
Functional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in mice.Anti-Human Antibody for Xenograft Studies
Lian Q, Zhang Y, Zhang J, Zhang HK, Wu X, Zhang Y, Lam FF, Kang S, Xia JC, Lai WH, Au KW, Chow YY, Siu CW, Lee CN, Tse HF (2010) Circulation.121:1113-23. Epub 2010 Feb 22.